An immunoassay based on lab-on-a-chip for simultaneous and sensitive detection of clenbuterol and ractopamine

Wang，Qi1,2; Deng，Jinqi2,4; Chen，Yiping2,5; Luo，Yunjing1; Jiang，Xingyu2,3,4

2020

10.1016/j.cclet.2020.01.028

CHINESE CHEMICAL LETTERS   影响因子和分区

1001-8417

1878-5964

Both clenbuterol (CLB) and ractopamine (RAC) are β-adrenergic agonists. After long-term excessive intake, there will be adverse reactions such as headache, chest tightness, limb numbness, and serious life-threatening. Simultaneous detection of CLB and RAC in related samples is of great importance for human health. In this work, we outline a microfluidics-based indirect competitive immunoassay (MICI) system that can sensitively detect residual CLB and RAC in pork, swine blood and swine urine. The rapid detection of multiple samples can be achieved in one chip, which greatly improves the detection efficiency. This method has good stability and reproducibility and the microfluidic chips are easy to manufacture. The linear ranges for CLB and RAC detection by MICI are 0.1–2.5 ng/mL and 0.1–5 ng/mL, and the limits of detection (LODs) are 0.094 ng/mL and 0.091 ng/mL, respectively. This straightforward and portable immunoassay system provides a good platform for rapid detection of harmful substances in food samples.

Clenbuterol Indirect competitive immunoassay Microfluidic chips Ractopamine Simultaneous detection

SCI

英语

其他

National Key R&D Program of China[2018YFA0902600][2017YFA0205901] ; National Natural Science Foundation of China[21535001][81730051][21761142006] ; Chinese Academy of Sciences[QYZDJ-SSW-SLH039][121D11KYSB20170026][XDA16020902]

Chemistry

Chemistry, Multidisciplinary

WOS:000548928800021

ELSEVIER SCIENCE INC

CHEMISTRY

2-s2.0-85078854755

Scopus

1.College of Life Science and Bioengineering,Beijing University of Technology,Beijing,100124,China
2.Beijing Engineering Research Center for BioNanotechnology and CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,CAS Center for Excellence in Nanoscience,National Center for NanoScience and Technology,Beijing,100190,China
3.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
4.University of Chinese Academy of Sciences,Beijing,100049,China
5.College of Food Science and Technology,Huazhong Agricultural University,Wuhan,430070,China

Wang，Qi,Deng，Jinqi,Chen，Yiping,et al. An immunoassay based on lab-on-a-chip for simultaneous and sensitive detection of clenbuterol and ractopamine[J]. CHINESE CHEMICAL LETTERS,2020,31(7):1835-1838.

Wang，Qi,Deng，Jinqi,Chen，Yiping,Luo，Yunjing,&Jiang，Xingyu.(2020).An immunoassay based on lab-on-a-chip for simultaneous and sensitive detection of clenbuterol and ractopamine.CHINESE CHEMICAL LETTERS,31(7),1835-1838.

Wang，Qi,et al."An immunoassay based on lab-on-a-chip for simultaneous and sensitive detection of clenbuterol and ractopamine".CHINESE CHEMICAL LETTERS 31.7(2020):1835-1838.