Drosophila YBX1 homolog YPS promotes ovarian germ line stem cell development by preferentially recognizing 5-methylcytosine RNAs

Zou，Fan1,2; Tu，Renjun3; Duan，Bo3; Yang，Zhenlin4; Ping，Zhaohua3; Song，Xiaoqing3; Chen，Shiyuan3; Price，Andrew3; Li，Hua3; Scott，Allison3; Perera，Anoja3; Li，Sisi2; Xie，Ting3,5

2020-02-18

10.1073/pnas.1910862117

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   影响因子和分区

0027-8424

1091-6490

5-Methylcytosine (mC) is a RNA modification that exists in tRNAs and rRNAs and was recently found in mRNAs. Although it has been suggested to regulate diverse biological functions, whether mC RNA modification influences adult stem cell development remains undetermined. In this study, we show that Ypsilon schachtel (YPS), a homolog of human Y box binding protein 1 (YBX1), promotes germ line stem cell (GSC) maintenance, proliferation, and differentiation in the Drosophila ovary by preferentially binding to mC-containing RNAs. YPS is genetically demonstrated to function intrinsically for GSC maintenance, proliferation, and progeny differentiation in the Drosophila ovary, and human YBX1 can functionally replace YPS to support normal GSC development. Highly conserved cold-shock domains (CSDs) of YPS and YBX1 preferentially bind to mC RNA in vitro. Moreover, YPS also preferentially binds to mC-containing RNAs, including mRNAs, in germ cells. The crystal structure of the YBX1 CSD-RNA complex reveals that both hydrophobic stacking and hydrogen bonds are critical for mC binding. Overexpression of RNA-binding–defective YPS and YBX1 proteins disrupts GSC development. Taken together, our findings show that mC RNA modification plays an important role in adult stem cell development.

GSC M5C RNA methylation YBX YPS

SCI

英语

NI期刊

通讯

NIH[R01 HD097664]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000514096400042

NATL ACAD SCIENCES

BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;PHARMACOLOGY & TOXICOLOGY;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;AGRICULTURAL SCIENCES;ENGINEERING

2-s2.0-85079531683

Scopus

1.Harbin Institute of Technology,Heilongjiang,150001,China
2.Department of Biology,Southern University of Science and Technology of China,Guangdong,518055,China
3.Stowers Institute for Medical Research,Kansas City,64110,United States
4.National Key Laboratory of Plant Molecular Genetics,Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences,Shanghai Center for Plant Stress Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai,201602,China
5.Department of Anatomy and Cell Biology,University of Kansas School of Medicine,Kansas City,66160,United States

Zou，Fan,Tu，Renjun,Duan，Bo,et al. Drosophila YBX1 homolog YPS promotes ovarian germ line stem cell development by preferentially recognizing 5-methylcytosine RNAs[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2020,117(7):3603-3609.

Zou，Fan.,Tu，Renjun.,Duan，Bo.,Yang，Zhenlin.,Ping，Zhaohua.,...&Xie，Ting.(2020).Drosophila YBX1 homolog YPS promotes ovarian germ line stem cell development by preferentially recognizing 5-methylcytosine RNAs.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,117(7),3603-3609.

Zou，Fan,et al."Drosophila YBX1 homolog YPS promotes ovarian germ line stem cell development by preferentially recognizing 5-methylcytosine RNAs".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117.7(2020):3603-3609.