A Small-Molecule Polycationic Crosslinker Boosts Alginate-Based Bioinks for Extrusion Bioprinting

Gao, Chongjian1,2; Tang, Lan1,3; Qu, Huawei1; Wu, Mingming1; Zhou, Tian4; Wen, Chunyi2; Wang, Pinpin1; Xu, Nan5,6; Ruan, Changshun1,3,5,6

2023-11-01

10.1002/adfm.202310369

ADVANCED FUNCTIONAL MATERIALS   影响因子和分区

1616-301X

1616-3028

["The importance of bioink suitability for extrusion bioprinting tissue-like constructs cannot be overemphasized. Owing to their excellent accessibility, alginate (Alg) -based bioinks are widely used. However, the printability accuracy and post-printing stability of current Alg-based bioinks remain challenging, especially for large-size fabrication. Herein, an improvement strategy for pre-crosslinking and post-crosslinking of methacrylated Alg (AlgMA) bioinks in extrusion bioprinting is presented via introduction of methacrylated e-polylysine (e-PLMA) as a small-molecule polycationic crosslinker. Due to their electrostatic interaction and covalent bonding, e-PLMA significantly reinforce the operability and prolong stability of AlgMA bioinks, in comparison with single-molecule Ca2+ or large-molecule methacrylated gelatin as pre-crosslinkers. Meanwhile, attributed to their regulation of a charged microenvironment (-345.25 to 121.55 mV) and hydrophilicity (26.64 degrees to 52.00 degrees), the cells in bioprinted AlgMA/PLMA constructs demonstrated improved viability and vitality. Therefore, such a strategy of introducing small-molecule cationic crosslinker e-PLMA is a promising booster for improving Alg-based extrusion bioprinting, potential to extend its biomedical applications.","methacrylated e-polylysine (e-PLMA), a small-molecule polycationic crosslinker, is introduced to formulate the alginate (Alg)-based polyelectrolyte bioinks with high printability for extrusion bioprinting. The fast mixture of small-molecule e-PLMA and methacrylated Alg (AlgMA) within 10 s significantly improves its operability, while their pre-printing viscosity, under-printing shear-thinning behaviors during extrusion, post-printing self-supporting behaviors and bioregulatory functions can be simply regulated by alteration of e-PLMA/AlgMA content ratio.image"]

alginate-based bioinks extrusion bioprinting printability shape maintenance e-polylysine

SCI ; EI

英语

通讯

National Natural Science Foundation of China["32122046","82072082","32000959","32101102","32201097"] ; Guangdong Natural Science Foundation[2020A1515111190] ; Shenzhen Fundamental Research Foundation["JCYJ20200109114006014","JCYJ20210324115814040","JCYJ20210324113001005","JCYJ20220531100602004"] ; National Key Clinical Specialty Construction Project[SZXK015] ; Guangdong High-level Clinical Key Specialties[SZGSP001] ; Shenzhen Science and Technology Program[JSGGKQTD20210831174330015] ; null[2022YFA1207502] ; null[2018YFA0703100]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:001108859400001

WILEY-V C H VERLAG GMBH

MATERIALS SCIENCE

Web of Science

1.Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Res Ctr Human Tissue & Organs Degenerat, Shenzhen 518055, Peoples R China
2.Hong Kong Polytech Univ, Dept Biomed Engn, Hung Hom, Hong Kong 999077, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Shenzhen Inst Adv Technol,Res Ctr Translat Med Res, Shenzhen 518055, Peoples R China
5.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Shenzhen 518020, Peoples R China
6.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Peoples R China

Gao, Chongjian,Tang, Lan,Qu, Huawei,et al. A Small-Molecule Polycationic Crosslinker Boosts Alginate-Based Bioinks for Extrusion Bioprinting[J]. ADVANCED FUNCTIONAL MATERIALS,2023.

Gao, Chongjian.,Tang, Lan.,Qu, Huawei.,Wu, Mingming.,Zhou, Tian.,...&Ruan, Changshun.(2023).A Small-Molecule Polycationic Crosslinker Boosts Alginate-Based Bioinks for Extrusion Bioprinting.ADVANCED FUNCTIONAL MATERIALS.

Gao, Chongjian,et al."A Small-Molecule Polycationic Crosslinker Boosts Alginate-Based Bioinks for Extrusion Bioprinting".ADVANCED FUNCTIONAL MATERIALS (2023).