Structural basis of nucleosomal H4K20 recognition and methylation by SUV420H1 methyltransferase

Lin，Folan1; Zhang，Ruxin2; Shao，Weihan1; Lei，Cong1; Ma，Mingxi1; Zhang，Ying1; Wen，Zengqi3; Li，Wanqiu1,4

2023-12-01

10.1038/s41421-023-00620-5

Cell Discovery   影响因子和分区

2056-5968

2056-5968

Histone lysine methyltransferase SUV420H1, which is responsible for site-specific di-/tri-methylation of histone H4 lysine 20 (H4K20), has crucial roles in DNA-templated processes, including DNA replication, DNA damage repair, and chromatin compaction. Its mutations frequently occur in human cancers. Nucleosomes containing the histone variant H2A.Z enhance the catalytic activities of SUV420H1 on H4K20 di-methylation deposition, regulating early replication origins. However, the molecular mechanism by which SUV420H1 specifically recognizes and deposits H4K20 methyl marks on nucleosomes remains poorly understood. Here we report the cryo-electron microscopy structures of SUV420H1 associated with H2A-containing nucleosome core particles (NCPs), and H2A.Z-containing NCPs. We find that SUV420H1 makes extensive site-specific contacts with histone and DNA regions. SUV420H1 C-terminal domain recognizes the H2A–H2B acidic patch of NCPs through its two arginine anchors, thus enabling H4K20 insertion for catalysis specifically. We also identify important residues increasing the catalytic activities of SUV420H1 bound to H2A.Z NCPs. In vitro and in vivo functional analyses reveal that multiple disease-associated mutations at the interfaces are essential for its catalytic activity and chromatin state regulation. Together, our study provides molecular insights into the nucleosome-based recognition and methylation mechanisms of SUV420H1, and a structural basis for understanding SUV420H1-related human disease.

SCI

英语

第一 ; 通讯

WOS:001113650800002

2-s2.0-85178462048

Scopus

1.Department of Pharmacology,Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,China
2.Institute of Molecular Physiology,Shenzhen Bay Laboratory,Shenzhen, Guangdong,China
3.School of Medicine,Shenzhen Campus of Sun Yat-Sen University,Sun Yat-Sen University,Shenzhen,Guangdong,China
4.Institute for Biological Electron Microscopy,Southern University of Science and Technology,Shenzhen,Guangdong,China

Lin，Folan,Zhang，Ruxin,Shao，Weihan,et al. Structural basis of nucleosomal H4K20 recognition and methylation by SUV420H1 methyltransferase[J]. Cell Discovery,2023,9(1).

Lin，Folan.,Zhang，Ruxin.,Shao，Weihan.,Lei，Cong.,Ma，Mingxi.,...&Li，Wanqiu.(2023).Structural basis of nucleosomal H4K20 recognition and methylation by SUV420H1 methyltransferase.Cell Discovery,9(1).

Lin，Folan,et al."Structural basis of nucleosomal H4K20 recognition and methylation by SUV420H1 methyltransferase".Cell Discovery 9.1(2023).