Arginine-Enhanced Antimicrobial Activity of Nanozymes against Gram-Negative Bacteria

Zhao, Zihan1,2,3,4,5,6; Wen, Shu'an1,2,3,4; Song, Ningning7; Wang, Lixiang8; Zhou, Yuan1,2,3,4; Deng, Xue1,2,3,4; Wu, Changbu1,2,3,4; Zhang, Guili1,2,3,4; Chen, Jun8; Tian, Guo-Bao1,2,3,4,9; Liang, Minmin7; Zhong, Lan-Lan1,2,3,4

2023-11-01

10.1002/adhm.202301332

ADVANCED HEALTHCARE MATERIALS   影响因子和分区

2192-2640

2192-2659

The continuous reduction of clinically available antibiotics has made it imperative to exploit more effective antimicrobial therapies, especially for difficult-to-treat Gram-negative pathogens. Herein, it is shown that the combination of an antimicrobial nanozyme with the clinically compatible basic amino acid L-arginine affords a potent treatment for infections with Gram-negative pathogens. In particular, the antimicrobial activity of the antimicrobial nanozyme is dramatically increased by approximate to 1000-fold after L-arginine stimulation. Specifically, the combination therapy enhances bacterial outer and inner membrane permeability and promotes intracellular reactive oxygen species (ROS) generation. Moreover, the metabolomic and transcriptomic results reveal that combination treatment leads to the increased ROS-mediated damage by inhibiting the tricarboxylic acid cycle and oxidative phosphorylation, thereby inducing an imbalance of the antioxidant and oxidant systems. Importantly, L-arginine dramatically significantly accelerates the healing of infected wounds in mouse models of multidrug-resistant peritonitis-sepsis and skin wound infection. Overall, this work demonstrates a novel synergistic antibacterial strategy by combining the antimicrobial nanozymes with L-arginine, which substantively facilitates the nanozyme-mediated killing of pathogens by promoting ROS production.

bacterial infections gram-negative pathogens nanozymes nanozyme adjuvants L-arginine

SCI ; EI

英语

其他

Guangdong Natural Science Foundation[2017A030306012] ; null[82325033] ; null[81830103] ; null[82061128001] ; null[82372294]

Engineering ; Science & Technology - Other Topics ; Materials Science

Engineering, Biomedical ; Nanoscience & Nanotechnology ; Materials Science, Biomaterials

WOS:001109966000001

WILEY

Web of Science

1.Sun Yat Sen Univ, Affiliated Hosp 5, Zhongshan Sch Med, Program Pathobiol, Guangzhou 510080, Guangdong, Peoples R China
2.Sun Yat sen Univ, Affiliated Hosp 1, Adv Med Technol Ctr, Zhongshan Sch Med, Guangzhou 510080, Peoples R China
3.Sun Yat sen Univ, Canc Ctr, State Key Lab Oncol South China, Guangzhou 510060, Peoples R China
4.Sun Yat Sen Univ, Key Lab Trop Dis Control, Minist Educ, Guangzhou 510080, Peoples R China
5.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Clin Lab, Shenzhen 518020, Peoples R China
6.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Peoples R China
7.Beijing Inst Technol, Expt Ctr Adv Mat, Sch Mat Sci & Engn, Beijing 100081, Peoples R China
8.Sun Yat Sen Univ, Zhongshan Sch Med, Dept Immunol & Microbiol, Guangzhou 510080, Peoples R China
9.Sun Yat Sen Univ, Sch Med, Dept Immunol, Shenzhen 518107, Peoples R China

Zhao, Zihan,Wen, Shu'an,Song, Ningning,et al. Arginine-Enhanced Antimicrobial Activity of Nanozymes against Gram-Negative Bacteria[J]. ADVANCED HEALTHCARE MATERIALS,2023.

Zhao, Zihan.,Wen, Shu'an.,Song, Ningning.,Wang, Lixiang.,Zhou, Yuan.,...&Zhong, Lan-Lan.(2023).Arginine-Enhanced Antimicrobial Activity of Nanozymes against Gram-Negative Bacteria.ADVANCED HEALTHCARE MATERIALS.

Zhao, Zihan,et al."Arginine-Enhanced Antimicrobial Activity of Nanozymes against Gram-Negative Bacteria".ADVANCED HEALTHCARE MATERIALS (2023).