Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy

Tang, Han1; Chen, Ji2; Qi, Lu He3; Lyu, Meng4; Quan, Hong1,5; Tan, Zhi Jie1,5

2023

10.2147/IJN.S422348

INTERNATIONAL JOURNAL OF NANOMEDICINE   影响因子和分区

1178-2013

Background: Photothermal therapy (PTT) has gained considerable interest as an emerging modality for cancer treatment in recent years. Radiation therapy (RT) has been widely used in the clinic as a traditional treatment method. However, RT and PTT treatments are limited by side effects and penetration depth, respectively. In addition, hypoxia within the tumor can lead to increased resistance to treatment.Methods: We synthesized multiple sizes of AuPt by modulating the reaction conditions. The smallest size of AuPt was selected and modified with folic acid (FA) for PTT and RT synergy therapy. Various methods including transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FITR) are used to determine the structure and composition of AuPt-FA (AF). In addition, we researched the photothermal properties of AF with IR cameras and infrared lasers. Flow cytometry, colony formation assays, CCK8, and fluorescent staining for probing the treatment effect in vitro. Also, we explored the targeting of AF by TEM and In Vivo Imaging Systems (IVIS). In vivo experiments, we record changes in tumor volume and weight as well as staining of tumor sections (ROS, Ki67, and hematoxylin and eosin).Results: The AuPt with particle size of 16 nm endows it with remarkably high photothermal conversion efficiency (46.84%) and catalase activity compared to other sizes of AuPt (30 nm and 100 nm). AF alleviates hypoxia in the tumor microenvironment, leading to the production of more reactive oxygen species (ROS) during the treatment. In addition, the therapeutic effect was significantly enhanced by combining RT and PTT, with an apoptosis rate of 81.1% in vitro and an in vivo tumor volume reduction rate of 94.0% in vivo. Conclusion: These results demonstrate that AF potentiates the synergistic effect of PTT and RT and has the potential for clinical translation.

photothermal radiotherapy hypoxia relief catalase activity microscale synergistic effect

SCI

英语

其他

National Science Foundation of China[11774272]

Science & Technology - Other Topics ; Pharmacology & Pharmacy

Nanoscience & Nanotechnology ; Pharmacology & Pharmacy

WOS:001108808400001

DOVE MEDICAL PRESS LTD

Web of Science

1.Wuhan Univ, Sch Phys & Technol, Minist Educ, Key Lab Artificial Microand Nanostruct, Wuhan, Peoples R China
2.Wuhan Univ, Zhongnan Hosp, Hubei Canc Clin Study Ctr, Hubei Key Lab Tumor Biol Behav,Dept Radiat & Med O, Wuhan, Peoples R China
3.Wuhan Univ, Sch Resource & Environm Sci, Wuhan, Peoples R China
4.Southern Univ Sci & Technol, Jinan Univ, Affiliated Hosp 1, Dept Gastrointestinal Surg,Shenzhen Peoples Hosp,C, Shenzhen, Guangdong, Peoples R China
5.Wuhan Univ, Sch Phys & Technol, Wuhan 430072, Hubei, Peoples R China

Tang, Han,Chen, Ji,Qi, Lu He,et al. Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2023,18.

Tang, Han,Chen, Ji,Qi, Lu He,Lyu, Meng,Quan, Hong,&Tan, Zhi Jie.(2023).Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy.INTERNATIONAL JOURNAL OF NANOMEDICINE,18.

Tang, Han,et al."Multifunctional AuPt Nanoparticles for Synergistic Photothermal and Radiation Therapy".INTERNATIONAL JOURNAL OF NANOMEDICINE 18(2023).