ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 in Vibrio parahaemolyticus

Zhang, Ce1,2; Liu, Min1,2; Wu, Ying1,2; Li, Xixi1,2; Zhang, Chen1,2; Call, Douglas R.3; Liu, Ming2,4; Zhao, Zhe1,2

2023-12-18

10.1080/19490976.2023.2281016

GUT MICROBES   影响因子和分区

1949-0976

1949-0984

In many Vibrio species, virulence is regulated by quorum sensing, which is regulated by a complex, multichannel, two-component phosphorelay circuit. Through this circuit, sensor kinases transmit sensory information to the phosphotransferase LuxU via a phosphotransfer mechanism, which in turn transmits the signal to the response regulator LuxO. For Vibrio parahaemolyticus, type III secretion system 1 (T3SS1) is required for cytotoxicity, but it is unclear how quorum sensing regulates T3SS1 expression. Herein, we report that a hybrid histidine kinase, ArcB, instead of LuxU, and sensor kinase LuxQ and response regulator LuxO, collectively orchestrate T3SS1 expression in V. parahaemolyticus. Under high oxygen conditions, LuxQ can interact with ArcB directly and phosphorylates the Hpt domain of ArcB. The Hpt domain of ArcB phosphorylates the downstream response regulator LuxO instead of ArcA. LuxO then activates transcription of the T3SS1 gene cluster. Under hypoxic conditions, ArcB autophosphorylates and phosphorylates ArcA, whereas ArcA does not participate in regulating the expression of T3SS1. Our data provides evidence of an alternative regulatory path involving the quorum sensing phosphorelay and adds another layer of understanding about the environmental regulation of gene expression in V. parahaemolyticus.

Quorum sensing type III secretion system ArcB two-component system

SCI

英语

通讯

National natural science foundation of china[31872597] ; Innovation Research Project for Marine Science and Technology of Jiangsu Province[JSZRHYKJ202209] ; Jiangsu Agricultural Science and Technology Independent Innovation Fund[CX [23]1007]

Gastroenterology & Hepatology ; Microbiology

Gastroenterology & Hepatology ; Microbiology

WOS:001108056000001

TAYLOR & FRANCIS INC

Web of Science

1.Hohai Univ, Jiangsu Prov Engn Res Ctr Marine Bioresources Sust, Nanjing, Peoples R China
2.Hohai Univ, Coll Oceanog, Dept Marine Biol, Nanjing, Peoples R China
3.Washington State Univ, Paul G Allen Sch Global Hlth, Pullman, WA USA
4.Southern Univ Sci & Technol, Hosp 2, Shenzhen Peoples Hosp 3, Dept Clin Lab,Natl Clin Res Ctr Infect Dis, Shenzhen, Guangdong, Peoples R China

Zhang, Ce,Liu, Min,Wu, Ying,et al. ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 in Vibrio parahaemolyticus[J]. GUT MICROBES,2023,15(2).

Zhang, Ce.,Liu, Min.,Wu, Ying.,Li, Xixi.,Zhang, Chen.,...&Zhao, Zhe.(2023).ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 in Vibrio parahaemolyticus.GUT MICROBES,15(2).

Zhang, Ce,et al."ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 in Vibrio parahaemolyticus".GUT MICROBES 15.2(2023).