南方科技大学知识苑(SUSTech KC): ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 in <i>Vibrio parahaemolyticus</i>

题名	ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 in Vibrio parahaemolyticus
作者	Zhang, Ce 1,2; Liu, Min 1,2; Wu, Ying 1,2; Li, Xixi 1,2; Zhang, Chen 1,2; Call, Douglas R.3; Liu, Ming 2,4 ; Zhao, Zhe 1,2
通讯作者	Liu, Ming; Zhao, Zhe
发表日期	2023-12-18
DOI	10.1080/19490976.2023.2281016
发表期刊	GUT MICROBES 影响因子和分区
ISSN	1949-0976
EISSN	1949-0984
卷号	15 期号:2
摘要	In many Vibrio species, virulence is regulated by quorum sensing, which is regulated by a complex, multichannel, two-component phosphorelay circuit. Through this circuit, sensor kinases transmit sensory information to the phosphotransferase LuxU via a phosphotransfer mechanism, which in turn transmits the signal to the response regulator LuxO. For Vibrio parahaemolyticus, type III secretion system 1 (T3SS1) is required for cytotoxicity, but it is unclear how quorum sensing regulates T3SS1 expression. Herein, we report that a hybrid histidine kinase, ArcB, instead of LuxU, and sensor kinase LuxQ and response regulator LuxO, collectively orchestrate T3SS1 expression in V. parahaemolyticus. Under high oxygen conditions, LuxQ can interact with ArcB directly and phosphorylates the Hpt domain of ArcB. The Hpt domain of ArcB phosphorylates the downstream response regulator LuxO instead of ArcA. LuxO then activates transcription of the T3SS1 gene cluster. Under hypoxic conditions, ArcB autophosphorylates and phosphorylates ArcA, whereas ArcA does not participate in regulating the expression of T3SS1. Our data provides evidence of an alternative regulatory path involving the quorum sensing phosphorelay and adds another layer of understanding about the environmental regulation of gene expression in V. parahaemolyticus.
关键词	Quorum sensing type III secretion system ArcB two-component system
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	National natural science foundation of china[31872597] ; Innovation Research Project for Marine Science and Technology of Jiangsu Province[JSZRHYKJ202209] ; Jiangsu Agricultural Science and Technology Independent Innovation Fund[CX [23]1007]
WOS研究方向	Gastroenterology & Hepatology ; Microbiology
WOS类目	Gastroenterology & Hepatology ; Microbiology
WOS记录号	WOS:001108056000001
出版者	TAYLOR & FRANCIS INC
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/629133
专题	南方科技大学第二附属医院南方科技大学第一附属医院
作者单位	1.Hohai Univ, Jiangsu Prov Engn Res Ctr Marine Bioresources Sust, Nanjing, Peoples R China 2.Hohai Univ, Coll Oceanog, Dept Marine Biol, Nanjing, Peoples R China 3.Washington State Univ, Paul G Allen Sch Global Hlth, Pullman, WA USA 4.Southern Univ Sci & Technol, Hosp 2, Shenzhen Peoples Hosp 3, Dept Clin Lab,Natl Clin Res Ctr Infect Dis, Shenzhen, Guangdong, Peoples R China
通讯作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Zhang, Ce,Liu, Min,Wu, Ying,et al. ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 in Vibrio parahaemolyticus[J]. GUT MICROBES,2023,15(2).
APA	Zhang, Ce.,Liu, Min.,Wu, Ying.,Li, Xixi.,Zhang, Chen.,...&Zhao, Zhe.(2023).ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 in Vibrio parahaemolyticus.GUT MICROBES,15(2).
MLA	Zhang, Ce,et al."ArcB orchestrates the quorum-sensing system to regulate type III secretion system 1 in Vibrio parahaemolyticus".GUT MICROBES 15.2(2023).