T-cell receptor sequencing reveals hepatocellular carcinoma immune characteristics according to Barcelona Clinic liver cancer stages within liver tissue and peripheral blood

Li, Rui1; Wang, Junxiao2,3; Li, Xiubin4; Liang, Yining1; Jiang, Yiyun1,5; Zhang, Yuwei; Xu, Pengfei6; Deng, Ling6; Wang, Zhe1; Sun, Tao6,7,12; Wu, Jian8,11; Xie, Hui2,10; Wang, Yijin1,9

2023-11-01

10.1111/cas.16013

CANCER SCIENCE   影响因子和分区

1347-9032

1349-7006

Analysis of T-cell receptor (TCR) repertoires in different stages of hepatocellular carcinoma (HCC) might help to elucidate its pathogenesis and progression. This study aimed to investigate TCR profiles in liver biopsies and peripheral blood mononuclear cells (PBMCs) in different Barcelona Clinic liver cancer (BCLC) stages of HCC. Ten patients in early stage (BCLC_A), 10 patients in middle stage (BCLC_B), and 10 patients in late stage (BCLC_C) cancer were prospectively enrolled. The liver tumor tissues, adjacent tissues, and PBMCs of each patient were collected and examined by TCR beta sequencing. Based on the ImMunoGeneTics (IMGT) database, we aligned the V, D, J, and C gene segments and identified the frequency of CDR3 sequences and amino acids sequence. Diversity of TCR in PBMCs was higher than in both tumor tissues and adjacent tissues, regardless of BCLC stage and postoperative recurrence. TCR clonality was increased in T cells from peripheral blood in advanced HCC, compared with the early and middle stages. No statistical differences were observed between different BCLC stages, either in tumors or adjacent tissues. TCR clonality revealed no significant difference between recurrent tumor and non-recurrent tumor, therefore PBMCs was better to be representative of TCR characteristics in different stages of HCC compared to tumor tissues. Clonal expansion of T cells was associated with low risk of recurrence in HCC patients.

clonal expansion HCC stages hepatocellular carcinoma recurrent tumor TCR sequencing

SCI

英语

第一 ; 通讯

Oncology

Oncology

WOS:001104063500001

WILEY

CLINICAL MEDICINE

Web of Science

1.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China
2.Chinese Peoples Liberat Army Gen Hosp, Med Ctr 5, Intervent Radiol, Beijing, Peoples R China
3.Peking Univ, Aerosp Ctr Hosp, Aerosp Med Ctr, Aerosp Clin Coll, Beijing, Peoples R China
4.Chinese Peoples Liberat Army Gen Hosp, Med Ctr 3, Dept Urol, Beijing, Peoples R China
5.Chinese Peoples Liberat Army Gen Hosp, Med Ctr 5, Dept Pathol & Hepatol, Beijing, Peoples R China
6.Hangzhou ImmuQuad Biotechnol, Hangzhou, Peoples R China
7.Zhejiang Univ, Inst Wenzhou, Wenzhou, Peoples R China
8.Nanjing Med Univ, Affiliated Suzhou Hosp, Suzhou Municipal Hosp, Gusu Sch,Dept Lab Med, Suzhou, Peoples R China
9.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Guangdong, Peoples R China
10.Fifth Med Ctr Chinese PLA Gen Hosp, Dept Invas Technol, Beijing 100039, Peoples R China
11.Nanjing Med Univ, Affiliated Suzhou Hosp, Suzhou Municipal Hosp, Gusu Sch,Dept Lab Med, Suzhou 215000, Peoples R China
12.Zhejiang Univ, Inst Wenzhou, Wenzhou 310000, Zhejiang, Peoples R China

Li, Rui,Wang, Junxiao,Li, Xiubin,et al. T-cell receptor sequencing reveals hepatocellular carcinoma immune characteristics according to Barcelona Clinic liver cancer stages within liver tissue and peripheral blood[J]. CANCER SCIENCE,2023.

Li, Rui.,Wang, Junxiao.,Li, Xiubin.,Liang, Yining.,Jiang, Yiyun.,...&Wang, Yijin.(2023).T-cell receptor sequencing reveals hepatocellular carcinoma immune characteristics according to Barcelona Clinic liver cancer stages within liver tissue and peripheral blood.CANCER SCIENCE.

Li, Rui,et al."T-cell receptor sequencing reveals hepatocellular carcinoma immune characteristics according to Barcelona Clinic liver cancer stages within liver tissue and peripheral blood".CANCER SCIENCE (2023).