PREDICTIVE VALUE OF NEUTROPHIL EXTRACELLULAR TRAP COMPONENTS FOR 28-DAY ALL-CAUSE MORTALITY IN PATIENTS WITH CARDIAC ARREST: A PILOT OBSERVATIONAL STUDY

Li, Peijuan1; Liang, Shuangshuang2; Wang, Ling1; Guan, Xiaolan1; Wang, Jin3,4; Gong, Ping1

2023-11-01

10.1097/SHK.0000000000002225

SHOCK   影响因子和分区

1073-2322

1540-0514

Background: Ischemia-reperfusion after cardiac arrest (CA) activates peptidyl arginine deiminase and citrullinated histone H3 (CitH3), which leads to the formation of neutrophil extracellular traps (NETs). This study attempted to determine the alterations in NET components in post-CA patients as well as analyze the association of NETs with 28-day all-cause mortality.Methods: In this study, 95 patients with restoration of spontaneous circulation (ROSC) after CA were included. They were categorized into the survivor group (n = 32) and the nonsurvivor group (n = 63) according to their 28-day survival statuses. The control group comprised 20 healthy individuals. The blood samples were collected from the patients on days 1, 3, and 7 after ROSC and from the control subjects at the time of enrollment. The serum cell-free DNA (cfDNA) level was determined using the fluorescent labeling method, and the serum concentrations of NET components, including CitH3, myeloperoxidase, neutrophil elastase, and nucleosomes, were estimated using the enzyme-linked immunosorbent assay. Results: Compared with the control group, the serum NET components were significantly increased in the patients 1 week after ROSC (all P < 0.05). These components were significantly higher in the nonsurvivor group than in the survivor group (all P < 0.05). Spearman correlational analysis revealed that the components were positively correlated with Acute Physiology and Chronic Health Evaluation II scores (both P < 0.05). Binary logistic regression analysis indicated that serum cfDNA, CitH3, and nucleosomes on days 1 and 3 after ROSC were independent predictors of 28-day all-cause mortality. Furthermore, these parameters on day 1 after ROSC had the biggest areas under the receiver operating characteristic curves (0.876, 0.862, and 0.861, respectively).Conclusions: Elevated serum levels of cfDNA, CitH3, myeloperoxidase, neutrophil elastase, and nucleosomes were positively correlated with disease severity after ROSC. However, only serum CitH3, cfDNA, and nucleosomes on day 1 after ROSC showed a good predictive value for 28-day all-cause mortality.

Cardiopulmonary resuscitation ischemia-reperfusion injury neutrophil extracellular traps prognosis

SCI

英语

通讯

Shenzhen Key Medical Discipline Construction Fund[SZXK046] ; National Natural Science Foundation of China[81571869]

General & Internal Medicine ; Hematology ; Surgery ; Cardiovascular System & Cardiology

Critical Care Medicine ; Hematology ; Surgery ; Peripheral Vascular Disease

WOS:001098543500005

LIPPINCOTT WILLIAMS & WILKINS

CLINICAL MEDICINE

Web of Science

1.Dalian Med Univ, Affiliated Hosp 1, Dept Emergency, 222 Zhongshan Rd, Dalian 116011, Liaoning, Peoples R China
2.Zhengzhou Univ, Zhengzhou Cent Hosp, Dept Emergency, Zhengzhou, Henan, Peoples R China
3.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Dept Emergency, 1017 Dongmen North Rd, Shenzhen 518020, Guangdong, Peoples R China
4.Southern Univ Sci & Technol, Affiliated Hosp 1, 1017 Dongmen North Rd, Shenzhen 518020, Guangdong, Peoples R China

Li, Peijuan,Liang, Shuangshuang,Wang, Ling,et al. PREDICTIVE VALUE OF NEUTROPHIL EXTRACELLULAR TRAP COMPONENTS FOR 28-DAY ALL-CAUSE MORTALITY IN PATIENTS WITH CARDIAC ARREST: A PILOT OBSERVATIONAL STUDY[J]. SHOCK,2023,60(5).

Li, Peijuan,Liang, Shuangshuang,Wang, Ling,Guan, Xiaolan,Wang, Jin,&Gong, Ping.(2023).PREDICTIVE VALUE OF NEUTROPHIL EXTRACELLULAR TRAP COMPONENTS FOR 28-DAY ALL-CAUSE MORTALITY IN PATIENTS WITH CARDIAC ARREST: A PILOT OBSERVATIONAL STUDY.SHOCK,60(5).

Li, Peijuan,et al."PREDICTIVE VALUE OF NEUTROPHIL EXTRACELLULAR TRAP COMPONENTS FOR 28-DAY ALL-CAUSE MORTALITY IN PATIENTS WITH CARDIAC ARREST: A PILOT OBSERVATIONAL STUDY".SHOCK 60.5(2023).