Lipidomic diversity and proxy implications of archaea from cold seep sediments of the South China Sea

Zhang, Tingting1,2,3; He, Wei4; Liang, Qianyong1,2,5; Zheng, Fengfeng4; Xiao, Xi1,2; Zeng, Zhiyu4; Zhou, Jingzhuo6; Yao, Wenyong4; Chen, Haodong7; Zhu, Yuanqing4,8; Zhao, Jing1; Zheng, Yan4,9; Zhang, Chuanlun4,5,8

2023-10-19

10.3389/fmicb.2023.1241958

FRONTIERS IN MICROBIOLOGY   影响因子和分区

1664-302X

Cold seeps on the continental margins are characterized by intense microbial activities that consume a large portion of methane by anaerobic methanotrophic archaea (ANME) through anaerobic oxidation of methane (AOM). Although ANMEs are known to contain unique ether lipids that may have an important function in marine carbon cycling, their full lipidomic profiles and functional distribution in particular cold-seep settings are still poorly characterized. Here, we combined the 16S rRNA gene sequencing and lipidomic approaches to analyze archaeal communities and their lipids in cold seep sediments with distinct methane supplies from the South China Sea. The archaeal community was dominated by ANME-1 in the moderate seepage area with strong methane emission. Low seepage area presented higher archaeal diversity covering Lokiarchaeia, Bathyarchaeia, and Thermoplasmata. A total of 55 core lipids (CLs) and intact polar lipids (IPLs) of archaea were identified, which included glycerol dialkyl glycerol tetraethers (GDGTs), hydroxy-GDGTs (OH-GDGTs), archaeol (AR), hydroxyarchaeol (OH-AR), and dihydroxyarchaeol (2OH-AR). Diverse polar headgroups constituted the archaeal IPLs. High concentrations of dissolved inorganic carbon (DIC) with depleted delta 13CDIC and high methane index (MI) values based on both CLs (MICL) and IPLs (MIIPL) indicate that ANMEs were active in the moderate seepage area. The ANME-2 and ANME-3 clades were characterized by enhanced glycosidic and phosphoric diether lipids production, indicating their potential role in coupling carbon and phosphurus cycling in cold seep ecosystems. ANME-1, though representing a smaller proportion of total archaea than ANME-2 and ANME-3 in the low seepage area, showed a positive correlation with MIIPL, indicating a different mechanism contributing to the IPL-GDGT pool. This also suggests that MIIPL could be a sensitive index to trace AOM activities performed by ANME-1. Overall, our study expands the understanding of the archaeal lipid composition in the cold seep and improves the application of MI using intact polar lipids that potentially link to extent ANME activities.

cold seeps South China Sea archaeal lipid biomarkers ANMEs methane index

SCI

英语

通讯

Financial supports for this research were provided by the National Natural Science Foundation of China (No. 42003063 to FZ and No. 42276087 to QL), the Marine Geological Survey Program of China Geological Survey (No. DD20221706 and DD20230065 to QL), the G["42003063","42276087"] ; National Natural Science Foundation of China["DD20221706","DD20230065"] ; Marine Geological Survey Program of China Geological Survey[2019B030302004] ; Guangdong Basic and Applied Basic Research Foundation[20200925173954005] ; Stable Support Plan Program of Shenzhen Natural Science Fund[ZDSYS201802081843490] ; Shenzhen Key Laboratory of Marine Archaea Geo-Omics, Southern University of Science and Technology[pdjh2021b0441]

Microbiology

Microbiology

WOS:001097667400001

FRONTIERS MEDIA SA

Web of Science

1.China Geol Survey, Guangzhou Marine Geol Survey, Guangzhou, Peoples R China
2.Natl Engn Res Ctr Gas Hydrate Explorat & Dev, Guangzhou, Peoples R China
3.Minist Nat Resources, East China Sea Ecol Ctr, Shanghai, Peoples R China
4.Southern Univ Sci & Technol, Dept Ocean Sci & Engn, Shenzhen Key Lab Marine Archaea Geoom, Shenzhen, Peoples R China
5.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou, Peoples R China
6.China Univ Min & Technol, Sch Chem Engn & Technol, Key Lab Coal Proc & Efficient Utilizat, Minist Educ, Xuzhou, Peoples R China
7.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou, Peoples R China
8.Shanghai Sheshan Natl Geophys Observ, Shanghai, Peoples R China
9.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Shenzhen, Peoples R China

Zhang, Tingting,He, Wei,Liang, Qianyong,et al. Lipidomic diversity and proxy implications of archaea from cold seep sediments of the South China Sea[J]. FRONTIERS IN MICROBIOLOGY,2023,14.

Zhang, Tingting.,He, Wei.,Liang, Qianyong.,Zheng, Fengfeng.,Xiao, Xi.,...&Zhang, Chuanlun.(2023).Lipidomic diversity and proxy implications of archaea from cold seep sediments of the South China Sea.FRONTIERS IN MICROBIOLOGY,14.

Zhang, Tingting,et al."Lipidomic diversity and proxy implications of archaea from cold seep sediments of the South China Sea".FRONTIERS IN MICROBIOLOGY 14(2023).