Biodegradation of Benzo[a]pyrene by a White-Rot Fungus Phlebia acerina: Surfactant-Enhanced Degradation and Possible Genes Involved

Zhang, Wenquan1; Li, Qiaoyu1; Wang, Jianqiao1; Wang, Ziyu1; Zhan, Hongjie1; Yu, Xiaolong2; Zheng, Yan2; Xiao, Tangfu1,3; Zhou, Li-Wei4

2023-10-01

10.3390/jof9100978

JOURNAL OF FUNGI   影响因子和分区

2309-608X

Polycyclic aromatic hydrocarbons (PAHs) are persistent environmental pollutants that pose a threat to human health. Among these PAHs, benzo[a]pyrene (BaP), a five-ring compound, exhibits high resistance to biodegradation. White-rot fungus Phlebia acerina S-LWZ20190614-6 has demonstrated higher BaP degradation capabilities compared with Phanerochaete chrysosporium and P. sordida YK-624, achieving a degradation rate of 57.7% after 32 days of incubation under a ligninolytic condition. To further enhance the biodegradation rate, three nonionic surfactants were used, and the addition of 1 or 2 gL-1 of polyethylene glycol monododecyl ether (Brij 30) resulted in nearly complete BaP biodegradation by P. acerina S-LWZ20190614-6. Interestingly, Brij 30 did not significantly affect the activity of manganese peroxidase and lignin peroxidase, but it did decrease laccase activity. Furthermore, the impact of cytochrome P450 on BaP degradation by P. acerina S-LWZ20190614-6 was found to be relatively mild. Transcriptomic analysis provided insights into the degradation mechanism of BaP, revealing the involvement of genes related to energy production and the synthesis of active enzymes crucial for BaP degradation. The addition of Brij 30 significantly upregulated various transferase and binding protein genes in P. acerina S-LWZ20190614-6. Hence, the bioremediation potential of BaP by the white-rot fungus P. acerina S-LWZ20190614-6 holds promise and warrants further exploration.

benzo[a]pyrene white-rot fungus degradation surfactants

SCI

英语

通讯

National Key R&D Program of China[2020YFC1807902] ; Basic Research Program of Guangzhou, China[202102010493] ; Science and Technology Program of Guangzhou[202102020950] ; Student Innovation Training Project[s202211078106]

Microbiology ; Mycology

Microbiology ; Mycology

WOS:001099501700001

MDPI

Web of Science

1.Guangzhou Univ, Minist Educ, Sch Environm Sci & Engn, Key Lab Water Qual & Conservat Pearl River Delta, Guangzhou 510006, Peoples R China
2.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Shenzhen 518055, Peoples R China
3.Chengdu Univ Technol, State Key Lab Geohazard Prevent & Geoenvironm Prot, Chengdu 610059, Peoples R China
4.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China

Zhang, Wenquan,Li, Qiaoyu,Wang, Jianqiao,et al. Biodegradation of Benzo[a]pyrene by a White-Rot Fungus Phlebia acerina: Surfactant-Enhanced Degradation and Possible Genes Involved[J]. JOURNAL OF FUNGI,2023,9(10).

Zhang, Wenquan.,Li, Qiaoyu.,Wang, Jianqiao.,Wang, Ziyu.,Zhan, Hongjie.,...&Zhou, Li-Wei.(2023).Biodegradation of Benzo[a]pyrene by a White-Rot Fungus Phlebia acerina: Surfactant-Enhanced Degradation and Possible Genes Involved.JOURNAL OF FUNGI,9(10).

Zhang, Wenquan,et al."Biodegradation of Benzo[a]pyrene by a White-Rot Fungus Phlebia acerina: Surfactant-Enhanced Degradation and Possible Genes Involved".JOURNAL OF FUNGI 9.10(2023).