Systematic thermal analysis of the Arabidopsis proteome: Thermal tolerance, organization, and evolution

Lyu, Hai-Ning1,2; Fu, Chunjin3; Chai, Xin1,2; Gong, Zipeng2,4; Zhang, Junzhe1,2; Wang, Jiaqi1,5; Wang, Jigang2,3,6,7; Dai, Lingyun3; Xu, Chengchao1,2,3

2023-10-18

10.1016/j.cels.2023.08.003

CELL SYSTEMS   影响因子和分区

2405-4712

2405-4720

Understanding the thermal stability of the plant proteome in the context of the native cellular environment would aid the design of crops with high thermal tolerance, but only limited such data are available. Here, we applied quantitative mass spectrometry to profile the thermal stability of the Arabidopsis proteome and identify thermo-sensitive and thermo-resilient protein networks in Arabidopsis, providing a basis for understanding heat-induced damage. We also show that the similarities of the protein-melting curves can be used as a proxy to evaluate system-wide protein-protein interactions in non-engineered plants and enable the identification of transient interactions exhibited by metabolons in the context of the cellular environment. Finally, we report a systematic comparison of the thermal stability of paralogs in Arabidopsis to aid the investigation and understanding of gene duplication and protein evolution. Taken together, our results could have broad implications for the fields of plant thermal tolerance, plant protein assemblies, and evolution.

SCI

英语

通讯

National Key R&D Program of China[2020YFA0908000] ; National Natural Science Foundation of China["82173914","32070748","32000026"] ; Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine[ZYYCXTD-C-202002] ; CACMS Innovation Fund["CI2021A05101","CI2021A05104"] ; Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences[CI2021B014] ; Fundamental Research Funds for the Central Public Welfare Research Institutes["ZZ14-YQ-051","ZZ14-YQ-052","ZZ14-ND-010","ZZ15-ND-10"] ; Excellent Scientific and Technological Innovation Training Program of Shenzhen[RCYX20210706092040048] ; State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University[FAMP202101K]

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:001096544200001

CELL PRESS

Web of Science

1.China Acad Chinese Med Sci, Artemisinin Res Ctr, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100700, Peoples R China
2.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Nephrol,Shenzhen Clin Res Ctr Geriatr, Shenzhen 518020, Peoples R China
4.Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Guizhou Prov Key Lab Pharmaceut, Guiyang 550004, Peoples R China
5.Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Shenzhen 518107, Peoples R China
6.Southern Med Univ, Sch Tradit Chinese Med, Guangzhou 510515, Peoples R China
7.Southern Med Univ, Sch Pharmaceut Sci, Guangzhou 510515, Peoples R China

Lyu, Hai-Ning,Fu, Chunjin,Chai, Xin,et al. Systematic thermal analysis of the Arabidopsis proteome: Thermal tolerance, organization, and evolution[J]. CELL SYSTEMS,2023,14(10).

Lyu, Hai-Ning.,Fu, Chunjin.,Chai, Xin.,Gong, Zipeng.,Zhang, Junzhe.,...&Xu, Chengchao.(2023).Systematic thermal analysis of the Arabidopsis proteome: Thermal tolerance, organization, and evolution.CELL SYSTEMS,14(10).

Lyu, Hai-Ning,et al."Systematic thermal analysis of the Arabidopsis proteome: Thermal tolerance, organization, and evolution".CELL SYSTEMS 14.10(2023).