Label-free microfluidic chip for segregation and recovery of circulating leukemia cells: clinical applications in acute myeloid leukemia

Ouyang，Dongfang1,2; Ye，Ningxin3; Jiang，Yue4; Wang，Yiyang5; Hu，Lina6; Chao，Shuen1,2; Yarmush，Martin1,2; Tuner，Memet1,2; Li，Yonghua7; Tang，Bin8

2024-03-01

10.1007/s10544-023-00687-7

Biomedical Microdevices   影响因子和分区

1387-2176

1572-8781

We present a label-free microfluidic chip for the segregation of circulating leukemia cells (CLCs) from blood samples, with a focus on its clinical applications in Acute Myeloid Leukemia (AML). The microfluidic chip achieved an approximate capture efficiency of 92%. The study analyzed a comprehensive set of 66 blood specimens from AML patients in different disease stages, including newly diagnosed and relapsing cases, patients in complete remission, and those in partial remission. The results showed a significant difference in CLC counts between active disease stages and remission stages (p < 0.0001), with a proposed threshold of 5 CLCs to differentiate between the two. The microfluidic chip exhibited a sensitivity of 95.4% and specificity of 100% in predicting disease recurrence. Additionally, the captured CLCs were subjected to downstream molecular analysis using droplet digital PCR, allowing for the identification of genetic mutations associated with AML. Comparative analysis with bone marrow aspirate processing by FACS demonstrated the reliability and accuracy of the microfluidic chip in tracking disease burden, with highly agreement results obtained between the two methods. The non-invasive nature of the microfluidic chip and its ability to provide real-time insights into disease progression make it a promising tool for the proactive monitoring and personalized patient care of AML.

Acute myeloid leukemia Circulating biomarkers Diagnosis Liquid biopsy Microfluidic chip

SCI

英语

其他

CLINICAL MEDICINE

2-s2.0-85179554044

Scopus

1.Center for Engineering in Medicine and Surgery,Massachusetts General Hospital,Harvard Medical School,Charlestown,02129,United States
2.Shriners Hospital for Children,Boston,02114,United States
3.Department of Biology,University of Ottawa,Ottawa,K1N 6N5,Canada
4.Medical Imaging Science,University of Manchester,Manchester,M13 9PL,United Kingdom
5.Department of Microbiology,Immunology and Molecular Genetics,University of Californiain,Los Angeles, Los Angeles,90095,United States
6.Department of Hematology,Shenzhen People’s Hospital,Shenzhen,Guangdong,518020,China
7.Department of Hematology,PLA General Hospital of Southern Theater Command,Guangzhou,Guangdong,510010,China
8.Department of Biomedical Engineering,South University of Science and Technology,Shenzhen,Guangdong,518055,China

Ouyang，Dongfang,Ye，Ningxin,Jiang，Yue,et al. Label-free microfluidic chip for segregation and recovery of circulating leukemia cells: clinical applications in acute myeloid leukemia[J]. Biomedical Microdevices,2024,26(1).

Ouyang，Dongfang.,Ye，Ningxin.,Jiang，Yue.,Wang，Yiyang.,Hu，Lina.,...&Tang，Bin.(2024).Label-free microfluidic chip for segregation and recovery of circulating leukemia cells: clinical applications in acute myeloid leukemia.Biomedical Microdevices,26(1).

Ouyang，Dongfang,et al."Label-free microfluidic chip for segregation and recovery of circulating leukemia cells: clinical applications in acute myeloid leukemia".Biomedical Microdevices 26.1(2024).