Direct dielectrophoretic characterization of particles in the high-density microwell array using optical tweezers

Luo，Xiao1,3; Wu，Chunhui2,4; Zhang，Jie5; Xu，Junyan2; Tan，Handong5; Zhang，Beini5; Xie，Jiesi6; Tao，Chaoran2; Huang，Kaicheng2; Cheng，Xing2,4; Wen，Weijia1,3,5

2024-03-01

10.1016/j.optlaseng.2023.107976

Optics and Lasers in Engineering   影响因子和分区

0143-8166

In this work, a quantitative and intuitive measurement of dielectrophoresis (DEP) behavior in a high-density dielectrophoretic microwell array chip was accomplished in assistance with the holographic optical tweezers. A one-dimensional force balance system with high reliability was achieved between the transverse optical trapping (TOT) force and the DEP force. The value of the DEP force was inferred by accurately calculating the TOT force, and the direction of the DEP force was observed with high-magnification microscopy. In order to estimate the availability and operability of the DEP force measurement system, we systematically investigated the influences of various external factors on the DEP behaviors of microspheres and live cells. All the quantitative measurement results, which agree well with the theory, showed that this system could serve as an accurate research tool for DEP behavior characterizations to optimize and improve the DEP-based manipulations of microparticles and live cells for a broad range of applications. The system also provides an approach to quantitatively obtain important parameters such as Clausius-Mossotti factors for cells in mediums, which could potentially be used to understand the dielectric properties of different types of cells for cell differentiation and diagnosis at the single-cell level.

Dielectrophoresis Microwell array chips Optical sensing Optical tweezers Optomechanics Single cell

SCI ; EI

英语

通讯

ENGINEERING

2-s2.0-85179133655

Scopus

1.Department of Physics,The Hong Kong University of Science and Technology,Hong Kong
2.Department of Materials Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
3.HKUST Shenzhen-Hong Kong Collaborative Innovation Research Institute,Futian,Shenzhen,518060,China
4.Academy for Advanced Interdisciplinary Studies,Southern University of Science and Technology,Shenzhen,518055,China
5.Advanced Materials Thrust,The Hong Kong University of Science and Technology (GZ),Guangzhou,511400,China
6.Guangzhou Kayja-Optics Technology Co.,Ltd.,Guangzhou,511400,China

Luo，Xiao,Wu，Chunhui,Zhang，Jie,et al. Direct dielectrophoretic characterization of particles in the high-density microwell array using optical tweezers[J]. Optics and Lasers in Engineering,2024,174.

Luo，Xiao.,Wu，Chunhui.,Zhang，Jie.,Xu，Junyan.,Tan，Handong.,...&Wen，Weijia.(2024).Direct dielectrophoretic characterization of particles in the high-density microwell array using optical tweezers.Optics and Lasers in Engineering,174.

Luo，Xiao,et al."Direct dielectrophoretic characterization of particles in the high-density microwell array using optical tweezers".Optics and Lasers in Engineering 174(2024).