A binary system based DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells

Li，Tong2; Sun，Mengxu2; Xia，Suping2; Huang，Ting2; Li，Rong Tian1; Li，Chunrong3; Dai，Zong5; Chen，Jin Xiang2; Chen，Jun2,4; Jia，Nuan1

2024-03-01

10.1016/j.talanta.2023.125465

Talanta   影响因子和分区

0039-9140

Developing simple, rapid and specific mRNA imaging strategy plays an important role in the early diagnosis of cancer and the new drugs development. Herein, we have established a novel binary system based DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This developed system consisted of tetrahedron probe A (TPA) and tetrahedron probe B (TPB). TK1 mRNA was chosen as the study model. After TPA and TPB enter into the live cells, the TK1 mRNA induces TPA and TPB to approach and activate the fluorescent aptamer, resulting in enhanced fluorescent signal in the presence of small molecules of DFHBI-1T. By this design, the high specificity label-free detection of nucleic acids was achieved with a detection limit of 1.34 nM. Confocal fluorescence imaging experiments had proved that this strategy could effectively distinguish the TK1 mRNA expression level between normal cell and cancer cell. The developed method is expected to provide a new tool for early diagnosis of diseases and new drug development.

Binary probes DNA tetrahedron Fluorogenic RNA aptamers mRNA imaging

SCI ; EI

英语

其他

CHEMISTRY

2-s2.0-85177746344

Scopus

1.Southern University of Science and Technology Hospital,Shenzhen,518055,China
2.NMPA Key Laboratory for Research and Evaluation of Drug Metabolism,Guangdong Provincial Key Laboratory of New Drug Screening,School of Pharmaceutical Sciences,Southern Medical University,Guangzhou,510515,China
3.Qiannan Medical College for Nationalities,Duyun,558000,China
4.Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science,Qingdao University of Science and Technology,Qingdao,MOE,266042,China
5.Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province,School of Biomedical Engineering,Sun Yat-Sen University,Shenzhen,518107,China

Li，Tong,Sun，Mengxu,Xia，Suping,et al. A binary system based DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells[J]. Talanta,2024,269.

Li，Tong.,Sun，Mengxu.,Xia，Suping.,Huang，Ting.,Li，Rong Tian.,...&Jia，Nuan.(2024).A binary system based DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells.Talanta,269.

Li，Tong,et al."A binary system based DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells".Talanta 269(2024).