The olfactory working memory capacity paradigm: A more sensitive and robust method of assessing cognitive function in male 5XFAD mice

Jiang，Li Xin1,2,3; Huang，Geng Di4,5; Wang，Hua Li1,2,3; Zhang，Chen6; Yu，Xin1,2,3

2024

10.1002/jnr.25265

Journal of Neuroscience Research   影响因子和分区

0360-4012

1097-4547

The olfactory working memory capacity (OWMC) paradigm is able to detect cognitive deficits in 5XFAD mice (an animal model of Alzheimer's disease [TG]) as early as 3 months of age, while other behavioral paradigms detect cognitive deficits only at 4–5 months of age. Therefore, we aimed to demonstrate that the OWMC paradigm is more sensitive and consistent in the early detection of declines in cognitive function than other commonly used behavioral paradigms. The prefrontal cortex (PFC), retrosplenial cortex (RSC), subiculum (SUB), and amygdala (AMY) of 5XFAD mice were harvested and subjected to immunostaining to detect the expression of β-amyloid (Aβ). Additionally, we compared the performance of 3-month-old male 5XFAD mice on common behavioral paradigms for assessing cognitive function (i.e., the open field [OF] test, novel object recognition [NOR] test, novel object location [NOL] test, Y-maze, and Morris water maze [MWM]) with that on the OWMC task. In the testing phase of the OWMC task, we varied the delay periods to evaluate the working memory capacity (WMC) of wild-type (WT) mice. Significant amyloid plaque deposition was observed in the PFC, RSC, SUB, and AMY of 3-month-old male 5XFAD mice. However, aside from the OWMC task, the other behavioral tests failed to detect cognitive deficits in 5XFAD mice. Additionally, to demonstrate the efficacy of the OWMC task in assessing WMC, we varied the retention delay periods; we found that the WMC of WT mice decreased with longer delay periods. The OWMC task is a sensitive and robust behavioral assay for detecting changes in cognitive function.

5XFAD mice Alzheimer's disease cognition RRID:AB_2056585 RRID:AB_2650602 RRID:AB_2935867 RRID:SCR_000441 RRID:SCR_002798 RRID:SCR_003070 RRID:SCR_013672 working memory capacity β-amyloid

SCI

英语

其他

NEUROSCIENCE & BEHAVIOR

2-s2.0-85176210007

Scopus

1.Peking University Institute of Mental Health (Sixth Hospital),Beijing,China
2.National Clinical Research Center for Mental Disorders & NHC Key Laboratory of Mental Health (Peking University),Beijing,China
3.Beijing Municipal Key Laboratory for Translational Research on Diagnosis and Treatment of Dementia,Beijing,China
4.Department of Addiction Medicine,Shenzhen Clinical Research Center for Mental Disorders,Shenzhen Mental Health Center,Shenzhen Kangning Hospital,Shenzhen,China
5.Affiliated Mental Health Center,Southern University of Science and Technology,Shenzhen,China
6.School of Basic Medical Sciences,Beijing Key Laboratory of Neural Regeneration and Repair,Advanced Innovation Center for Human Brain Protection,Capital Medical University,Beijing,China

Jiang，Li Xin,Huang，Geng Di,Wang，Hua Li,et al. The olfactory working memory capacity paradigm: A more sensitive and robust method of assessing cognitive function in male 5XFAD mice[J]. Journal of Neuroscience Research,2024,102(1).

Jiang，Li Xin,Huang，Geng Di,Wang，Hua Li,Zhang，Chen,&Yu，Xin.(2024).The olfactory working memory capacity paradigm: A more sensitive and robust method of assessing cognitive function in male 5XFAD mice.Journal of Neuroscience Research,102(1).

Jiang，Li Xin,et al."The olfactory working memory capacity paradigm: A more sensitive and robust method of assessing cognitive function in male 5XFAD mice".Journal of Neuroscience Research 102.1(2024).