High-speed adaptive photoacoustic microscopy

Li，Linyang1; Qin，Wei1; Li，Tingting1; Zhang，Junning1; Li，Baochen1; Xi，Lei1,2,3

2023-12-01

10.1364/PRJ.499598

Photonics Research   影响因子和分区

2327-9125

Optical-resolution photoacoustic microscopy (OR-PAM) is capable of observing the distribution of optical absorbers inside bio-tissues with a high spatial resolution of micrometers. Unfortunately, due to the employment of a tight optical focus, it suffers from a limited depth of field (DOF), making it challenging to achieve highresolution imaging of targets with arbitrary surfaces. Here, we propose a high spatiotemporal adaptive photoacoustic focusing mechanism through integrating a high-speed optical focuser, a time-of-flight contour deriving algorithm, and the rotary-scanning photoacoustic microscopy. The developed system, named high-speed adaptive photoacoustic microscopy (HA-PAM), features an ultrashort focus-shifting time of 5 ms and an enlarged DOF of up to 5 mm. With the assistance of the proposed mechanism, we can achieve a homogeneous lateral resolution of 6 μm over a 10 mm circular imaging domain within 5 s. We demonstrate the advantages of HA-PAM through imaging phantoms with curved surfaces, subcutaneous tumor-bearing mice, resected rabbit kidneys, and pulsating mouse brains. The imaging results suggest that this approach provides a high and consistent spatial resolution for imaging bio-tissues with arbitrary surfaces without sacrificing the imaging speed, and has the potential to extend the fundamental and clinical applications of OR-PAM.

EI ; SCI

英语

第一 ; 通讯

20234915184100

Histology ; Image resolution ; Mammals ; Tissue

Biological Materials and Tissue Engineering:461.2 ; Imaging Techniques:746

2-s2.0-85179000454

Scopus

1.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
2.Guangdong Provincial Key Laboratory of Advanced Biomaterials,Southern University of Science and Technology,Shenzhen,518055,China
3.Shenzhen Bay Laboratory,Shenzhen,518132,China

Li，Linyang,Qin，Wei,Li，Tingting,et al. High-speed adaptive photoacoustic microscopy[J]. Photonics Research,2023,11(12):2084-2092.

Li，Linyang,Qin，Wei,Li，Tingting,Zhang，Junning,Li，Baochen,&Xi，Lei.(2023).High-speed adaptive photoacoustic microscopy.Photonics Research,11(12),2084-2092.

Li，Linyang,et al."High-speed adaptive photoacoustic microscopy".Photonics Research 11.12(2023):2084-2092.