Synthetic Biohybrids of Red Blood Cells and Cascaded-Enzymes@ Metal-Organic Frameworks for Hyperuricemia Treatment

Li, Zeyu1; Xue, Liecong1; Yang, Junxian2; Wuttke, Stefan3,4; He, Peiying1; Lei, Chuanyi1; Yang, Haowei5; Zhou, Liang1; Cao, Jiangfan1; Sinelshchikova, Anna3; Zheng, Guansheng1; Guo, Jimin6; Lin, Jiangguo2; Lei, Qi7; Brinker, C. Jeffrey8,9; Liu, Kaisheng10,11; Zhu, Wei1

2023-12-01

10.1002/advs.202305126

ADVANCED SCIENCE   影响因子和分区

2198-3844

["Hyperuricemia, caused by an imbalance between the rates of production and excretion of uric acid (UA), may greatly increase the mortality rates in patients with cardiovascular and cerebrovascular diseases. Herein, for fast-acting and long-lasting hyperuricemia treatment, armored red blood cell (RBC) biohybrids, integrated RBCs with proximal, cascaded-enzymes of urate oxidase (UOX) and catalase (CAT) encapsulated within ZIF-8 framework-based nanoparticles, have been fabricated based on a super-assembly approach. Each component is crucial for hyperuricemia treatment: 1) RBCs significantly increase the circulation time of nanoparticles; 2) ZIF-8 nanoparticles-based superstructure greatly enhances RBCs resistance against external stressors while preserving native RBC properties (such as oxygen carrying capability); 3) the ZIF-8 scaffold protects the encapsulated enzymes from enzymatic degradation; 4) no physical barrier exists for urate diffusion, and thus allow fast degradation of UA in blood and neutralizes the toxic by-product H2O2. In vivo results demonstrate that the biohybrids can effectively normalize the UA level of an acute hyperuricemia mouse model within 2 h and possess a longer elimination half-life (49.7 +/- 4.9 h). They anticipate that their simple and general method that combines functional nanomaterials with living cell carriers will be a starting point for the development of innovative drug delivery systems.","Armored red blood cell (RBC) biohybrids, integrated RBCs with proximal, cascaded-enzymes of urate oxidase and catalase encapsulated within ZIF-8 framework-based nanoparticles, have been fabricated for fast-acting and long-lasting urate-lowering therapy. RBC biohybrids could normalize the UA level of acute hyperuricemia model mice within 2 h, and possessed an elimination half-life of 49.7 +/- 4.9 h, which is over thrice longer than that of free UOX, representing an innovative drug carrier for hyperuricemia treatment.image"]

biohybrid materials red blood cells Metal-organic Frameworks enzyme hyperuricemia

SCI ; EI

英语

通讯

National Natural Science Foundation of China["22372061","21972047"] ; Natural Science Foundation of Guangdong Province, China["2021A1515010724","2021A1515220051","2022A0505050008"] ; Guangdong Provincial Pearl River Talents Program[2019QN01Y314] ; Program for Guangdong Introducing Innovative and Entrepreneurial Teams[2019ZT08Y318] ; Science and Technology Project of Guangzhou, China["202102020352","202102020259"]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:001113504900001

WILEY

2-s2.0-85178434152

Web of Science

1.South China Univ Technol, Sch Biol & Biol Engn, MOE Int Joint Res Lab Synthet Biol & Med, Guangzhou 510006, Peoples R China
2.Southern Med Univ, Guangdong Acad Med Sci, Med Res Inst, Guangdong Prov Peoples Hosp, Guangzhou 510080, Peoples R China
3.UPV EHU Sci Pk, Basque Ctr Mat, BCMaterials, Leioa 48940, Spain
4.IKERBASQUE, Basque Fdn Sci, Bilbao 48009, Spain
5.China Natl Tobacco Corp, 55 South Yuetan Blvd, Beijing 100045, Peoples R China
6.Beijing Univ Chem Technol, Coll Mat Sci & Engn, Beijing 100029, Peoples R China
7.Guangzhou Med Univ, Affiliated Hosp 2, State Key Lab Resp Dis, Guangdong Prov Key Lab Allergy & Clin Immunol, Guangzhou 510260, Peoples R China
8.Univ New Mexico, Ctr Microengn Mat, Albuquerque, NM 87131 USA
9.Univ New Mexico, Dept Chem & Biol Engn, Albuquerque, NM 87131 USA
10.Jinan Univ, Shenzhen Peoples Hosp, Guangdong Prov Clin Res Ctr Geriatr, Clin Med Coll 2,Shenzhen Clin Res Ctr Geriatr, Shenzhen 518020, Peoples R China
11.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Peoples R China

Li, Zeyu,Xue, Liecong,Yang, Junxian,et al. Synthetic Biohybrids of Red Blood Cells and Cascaded-Enzymes@ Metal-Organic Frameworks for Hyperuricemia Treatment[J]. ADVANCED SCIENCE,2023,11(5).

Li, Zeyu.,Xue, Liecong.,Yang, Junxian.,Wuttke, Stefan.,He, Peiying.,...&Zhu, Wei.(2023).Synthetic Biohybrids of Red Blood Cells and Cascaded-Enzymes@ Metal-Organic Frameworks for Hyperuricemia Treatment.ADVANCED SCIENCE,11(5).

Li, Zeyu,et al."Synthetic Biohybrids of Red Blood Cells and Cascaded-Enzymes@ Metal-Organic Frameworks for Hyperuricemia Treatment".ADVANCED SCIENCE 11.5(2023).