Multi-scale mechanical investigation of articular cartilage suffered progressive pseudorheumatoid dysplasia

Lin，L. J.1; Ge，Y. M.2; Tian，Y.3; Liu，N.2; Luo，X. H.2; Xue，Y. T.2; Xue，Y. Z.B.2; Wen，C. Y.4; Tang，B.2,3

2020

10.1016/j.clinbiomech.2019.12.029

CLINICAL BIOMECHANICS   影响因子和分区

0268-0033

1879-1271

Background: Progressive pseudorheumatoid dysplasia is a rare skeletal dysplasia mainly caused by abnormal autosomal recessive inheritance. Although the main function of cartilage is mechanical support and the characteristics of this disease is the degradation of AC, previous studies on it had been mainly focused on clinical and genetic aspects and the mechanical behavior of the cartilage affected by PPRD is still ambiguous. In this study, we investigate the mechanics and structure of the cartilage suffered disease at multi-scale, from individual chondrocytes to the bulk-scale tissue. Methods: Depth-sensing indenter were employed to investigate the mechanics of cartilage; we performed atomic force microscope nanoindentation to investigate the cell mechanics and scanning electron microscopy were used to explore the structure feature and chemical composition. Findings: The elastic modulus of chondrocytes harvested from cartilage suffered from progressive pseudorheumatoid dysplasia is significantly higher than from normal cartilage, same trend were also found in tissue level. Moreover, denser collagen meshwork and matrix calcification were also observed. Interpretation: The elastic modulus of cartilage should closely related to its denser structure and the calcification, and may potentially be an indicator for clinical diagnosis. The stiffening of chondrocytes during PPRD progression should play a rather important role in its pathogenesis.

Atomic force microscope Cartilage Chondrocyte Nanoindentation PPRD

SCI ; EI

英语

通讯

National Foundation of Science and Technology[11872200]

Engineering ; Orthopedics ; Sport Sciences

Engineering, Biomedical ; Orthopedics ; Sport Sciences

WOS:000579839200006

ELSEVIER SCI LTD

20200308045390

Biomineralization ; Diagnosis ; Scanning electron microscopy ; Bone ; Elastic moduli ; Nanoindentation ; Atomic force microscopy

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biology:461.9 ; Optical Devices and Systems:741.3 ; Nanotechnology:761 ; Biochemistry:801.2 ; Mechanical Variables Measurements:943.2 ; Materials Science:951

CLINICAL MEDICINE

2-s2.0-85077917397

Scopus

1.Department of Orthopedics,Zhujiang Hospital,Southern Medical University,Guangzhou,China
2.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,China
3.Department of Materials Science and Engineering,Southern University of Science and Technology,Shenzhen,China
4.Interdisciplinary Division of Biomedical Engineering,Hong Kong Polytechnic University,China

Lin，L. J.,Ge，Y. M.,Tian，Y.,et al. Multi-scale mechanical investigation of articular cartilage suffered progressive pseudorheumatoid dysplasia[J]. CLINICAL BIOMECHANICS,2020,79.

Lin，L. J..,Ge，Y. M..,Tian，Y..,Liu，N..,Luo，X. H..,...&Tang，B..(2020).Multi-scale mechanical investigation of articular cartilage suffered progressive pseudorheumatoid dysplasia.CLINICAL BIOMECHANICS,79.

Lin，L. J.,et al."Multi-scale mechanical investigation of articular cartilage suffered progressive pseudorheumatoid dysplasia".CLINICAL BIOMECHANICS 79(2020).