Unravelling biosynthesis and biodegradation potentials of microbial dark matters in hypersaline lakes

Qiu，Zhiguang1,2; Zhu，Yuanyuan1; Zhang，Qing1; Qiao，Xuejiao1; Mu，Rong1; Xu，Zheng3,4; Yan，Yan5; Wang，Fan6; Zhang，Tong7; Zhuang，Wei Qin8; Yu，Ke1,2

2024-07-01

10.1016/j.ese.2023.100359

Environmental Science and Ecotechnology   影响因子和分区

2666-4984

Biosynthesis and biodegradation of microorganisms critically underpin the development of biotechnology, new drugs and therapies, and environmental remediation. However, most uncultured microbial species along with their metabolic capacities in extreme environments, remain obscured. Here we unravel the metabolic potential of microbial dark matters (MDMs) in four deep-inland hypersaline lakes in Xinjiang, China. Utilizing metagenomic binning, we uncovered a rich diversity of 3030 metagenome-assembled genomes (MAGs) across 82 phyla, revealing a substantial portion, 2363 MAGs, as previously unclassified at the genus level. These unknown MAGs displayed unique distribution patterns across different lakes, indicating a strong correlation with varied physicochemical conditions. Our analysis revealed an extensive array of 9635 biosynthesis gene clusters (BGCs), with a remarkable 9403 being novel, suggesting untapped biotechnological potential. Notably, some MAGs from potentially new phyla exhibited a high density of these BGCs. Beyond biosynthesis, our study also identified novel biodegradation pathways, including dehalogenation, anaerobic ammonium oxidation (Anammox), and degradation of polycyclic aromatic hydrocarbons (PAHs) and plastics, in previously unknown microbial clades. These findings significantly enrich our understanding of biosynthesis and biodegradation processes and open new avenues for biotechnological innovation, emphasizing the untapped potential of microbial diversity in hypersaline environments.

Biodegradation Biosynthesis Hypersaline lakes Metagenome-assembled genomes Microbial dark matters

SCI

英语

其他

2-s2.0-85181242770

Scopus

1.School of Environment and Energy,Peking University Shenzhen Graduate School,Shenzhen,518055,China
2.AI for Science (AI4S)-Preferred Program,Peking University,Shenzhen,518055,China
3.Southern University of Sciences and Technology Yantian Hospital,Shenzhen,518081,China
4.Institute of Biomedicine and Biotechnology,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China
5.State Key Laboratory of Isotope Geochemistry,CAS Center for Excellence in Deep Earth Science,Guangzhou Institute of Geochemistry,Chinese Academy of Sciences,Guangzhou,510640,China
6.School of Atmospheric Sciences,Sun Yat-sen University,Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai),Zhuhai,519082,China
7.Department of Civil Engineering,University of Hong Kong,999077,Hong Kong
8.Department of Civil and Environmental Engineering,Faculty of Engineering,University of Auckland,New Zealand

Qiu，Zhiguang,Zhu，Yuanyuan,Zhang，Qing,et al. Unravelling biosynthesis and biodegradation potentials of microbial dark matters in hypersaline lakes[J]. Environmental Science and Ecotechnology,2024,20.

Qiu，Zhiguang.,Zhu，Yuanyuan.,Zhang，Qing.,Qiao，Xuejiao.,Mu，Rong.,...&Yu，Ke.(2024).Unravelling biosynthesis and biodegradation potentials of microbial dark matters in hypersaline lakes.Environmental Science and Ecotechnology,20.

Qiu，Zhiguang,et al."Unravelling biosynthesis and biodegradation potentials of microbial dark matters in hypersaline lakes".Environmental Science and Ecotechnology 20(2024).