DeKinomics pulse-chases kinase functions in living cells

Weng，Yicheng1,2,3,4,5; Chen，Wendong2,4,6; Kong，Qian2,4; Wang，Ruixiang1,3,5; Zeng，Ruxin1; He，An2,4; Liu，Yanjun1; Mao，Yiheng2,4; Qin，Yunqiu2,4; Ngai，William Shu Ching7; Zhang，Heng7; Ke，Mi2,4; Wang，Jie2; Tian，Ruijun2,4; Chen，Peng R.1,3,5,7

2024

10.1038/s41589-023-01497-x

Nature Chemical Biology   影响因子和分区

1552-4450

1552-4469

Cellular context is crucial for understanding the complex and dynamic kinase functions in health and disease. Systematic dissection of kinase-mediated cellular processes requires rapid and precise stimulation (‘pulse’) of a kinase of interest, as well as global and in-depth characterization (‘chase’) of the perturbed proteome under living conditions. Here we developed an optogenetic ‘pulse-chase’ strategy, termed decaging kinase coupled proteomics (DeKinomics), for proteome-wide profiling of kinase-driven phosphorylation at second-timescale in living cells. We took advantage of the ‘gain-of-function’ feature of DeKinomics to identify direct kinase substrates and further portrayed the global phosphorylation of understudied receptor tyrosine kinases under native cellular settings. DeKinomics offered a general activation-based strategy to study kinase functions with high specificity and temporal resolution under living conditions. [Figure not available: see fulltext.].

SCI

英语

其他

2-s2.0-85181212004

Scopus

1.New Cornerstone Science Laboratory,Synthetic and Functional Biomolecules Center,Beijing National Laboratory for Molecular Sciences,New Cornerstone Science Laboratory,College of Chemistry and Molecular Engineering,Peking University,Beijing,China
2.Department of Chemistry and Research Center for Chemical Biology and Omics Analysis,School of Science,Southern University of Science and Technology,Shenzhen,China
3.Academy for Advanced Interdisciplinary Studies,Peking University,Beijing,China
4.Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research,Southern University of Science and Technology,Shenzhen,China
5.Peking-Tsinghua Center for Life Sciences,Peking University,Beijing,China
6.South China Institute of Biomedicine,Academy of Phronesis Medicine,Guangzhou,China
7.Shenzhen Bay Laboratory,Shenzhen,China

Weng，Yicheng,Chen，Wendong,Kong，Qian,et al. DeKinomics pulse-chases kinase functions in living cells[J]. Nature Chemical Biology,2024.

Weng，Yicheng.,Chen，Wendong.,Kong，Qian.,Wang，Ruixiang.,Zeng，Ruxin.,...&Chen，Peng R..(2024).DeKinomics pulse-chases kinase functions in living cells.Nature Chemical Biology.

Weng，Yicheng,et al."DeKinomics pulse-chases kinase functions in living cells".Nature Chemical Biology (2024).