| 题名 | Photoswitchable vibrational nanoscopy with sub-100-nm optical resolution |
| 作者 | |
| 通讯作者 | Ji, Minbiao |
| 发表日期 | 2023-11-01
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| DOI | |
| 发表期刊 | |
| EISSN | 2577-5421
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| 卷号 | 5期号:6 |
| 摘要 | Stimulated Raman scattering (SRS) microscopy has shown superior chemical resolution due to the much narrower vibrational spectral bandwidth than its fluorescence counterpart. However, breaking the diffraction-limited spatial resolution of SRS imaging is much more challenging because of the intrinsically weak scattering cross section and inert/stable nature of molecular bond vibrations. We report superresolution SRS (SR-SRS) nanoscopy based on reversible-switchable vibrational photochromic probes integrated with point spread function engineering strategy. By introducing a Gaussian-shaped ultraviolet excitation beam and a donut-shaped visible depletion beam in addition to the pump and Stokes beams, SR-SRS could reach sub-100 nm resolution on photoswitchable nanoparticles (NPs). Furthermore, NP-treated live cell imaging was demonstrated with resolution improvement by a factor of ∼4. Our proof-of-principle work provides the potential for SR vibrational imaging to assist research on complex biological systems. © The Authors. |
| 关键词 | |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
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| 学校署名 | 其他
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| 资助项目 | We would like to thank P. Wang for the kind provision of SM fiber and H. Yan for the support of FTIR measurement. M. Ji would like to acknowledge the financial support from the National Key Research and Development Program of China (Grant No. 2021YFF0502900), the National Natural Science Foundation of China (Grant Nos. 61975033 and 81771930), the Shanghai Municipal Science and Technology Major Project (Grant Nos. 2017SHZDZX01 and 2018SHZDZX01), and ZJLab. C. Wu would like to acknowledge the financial support from the Shenzhen Science and Technology Innovation Commission (Grant No. KQTD20170810111314625) and the National Key Research and Development Program of China (Grant No. 2018YFB0407200).
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| WOS研究方向 | Optics
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| WOS类目 | Optics
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| WOS记录号 | WOS:001130399500003
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| 出版者 | |
| EI入藏号 | 20235215285927
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| EI主题词 | Optical resolving power
; Optical transfer function
; Stimulated Raman scattering
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| EI分类号 | Electromagnetic Waves:711
; Light/Optics:741.1
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| 来源库 | EV Compendex
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| 引用统计 |
被引频次[WOS]：8
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/673935 |
| 专题 | 工学院_生物医学工程系 |
| 作者单位 | 1.Fudan University, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Academy for Engineering and Technology, Department of Physics, State Key Laboratory of Surface Physics, Shanghai, China 2.Southern University of Science and Technology, Department of Biomedical Engineering, Shenzhen, China |
| 推荐引用方式 GB/T 7714 |
Ao, Jianpeng,Fang, Xiaofeng,Ma, Liyang,et al. Photoswitchable vibrational nanoscopy with sub-100-nm optical resolution[J]. Advanced Photonics,2023,5(6).
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| APA |
Ao, Jianpeng.,Fang, Xiaofeng.,Ma, Liyang.,Liu, Zhijie.,Wu, Simin.,...&Ji, Minbiao.(2023).Photoswitchable vibrational nanoscopy with sub-100-nm optical resolution.Advanced Photonics,5(6).
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| MLA |
Ao, Jianpeng,et al."Photoswitchable vibrational nanoscopy with sub-100-nm optical resolution".Advanced Photonics 5.6(2023).
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| 条目包含的文件 | 条目无相关文件。 | |||||
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