南方科技大学知识苑(SUSTech KC): Orientational Nanoconjugation with Gold Endows Marked Antimicrobial Potential and Drugability of Ultrashort Dipeptides

题名	Orientational Nanoconjugation with Gold Endows Marked Antimicrobial Potential and Drugability of Ultrashort Dipeptides
作者	Zhang, Zhiye 1; Chen, Yaoyao 2; Gao, Jinai 1,3; Yang, Min 1,8; Zhang, Dengdeng 1,4; Wang, Le5 ; Zhang, Tianyu 6; Cao, Qiqi 2; Mwangi, James 1,8; He, Chenglu 7; Li, Ya 7; Liu, Xiangsheng 6; Jiang, Xingyu5 ; Kamau, Peter Muiruri 1,8; Lai, Ren 1,3
通讯作者	Lai, Ren
发表日期	2023
DOI	10.1021/acs.nanolett.3c03909
发表期刊	Nano Letters 影响因子和分区
ISSN	1530-6984
EISSN	1530-6992
卷号	23 期号:24
摘要	Antibiotic resistance is a global threat. Antimicrobial peptides (AMPs) are highly desirable to treat multidrug-resistant pathogen infection. However, few AMPs are clinically available, due to high cost, instability, and poor selectivity. Here, ultrashort AMPs (2-3 residues with an N-terminal cysteine) are designed and assembled as gold nanoparticles. Au-S conjugation and ultrashort size restrict nonspecific reactions and peptide orientation, thus concentrating positively charged residues on the surface. The nanostructured assemblies enormously enhance antimicrobial abilities by 1000-6000-fold and stability. One representative (Au-Cys-Arg-NH2, Au_CR) shows selective antibacterial activity against Staphylococcus aureus with 10 nM minimal inhibitory concentration. Au_CR has comparable or better in vivo antimicrobial potency than vancomycin and methicillin, with low propensity to induce resistance, little side effects, and high stability (17.5 h plasma half-life). Au_CR acts by inducing collapse of membrane potential and rupture of the bacterial membrane. The report provides insights for developing AMP-metal nanohybrids, particularly tethering nonspecific reactions and AMP orientation on the metal surface. © 2023 The Authors. Published by American Chemical Society.
关键词	dipeptide gold nanoparticles antibiotic-resistance antimicrobial peptides
相关链接	[来源记录]
收录类别	EI ; SCI
语种	英语
学校署名	其他
资助项目	This work was supported by the National Natural Science Foundation of China (32070443 and 21761142002), Chinese Academy of Sciences (XDB31000000, SAJC201606, KFJ-BRP-008, and KGFZD-135-17-011), and Yunnan Province Grant (202302AA310015).
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS记录号	WOS:001133414000001
出版者	American Chemical Society
EI入藏号	20240115314927
EI主题词	Amino acids ; Antibiotics ; Bacteria ; Fiber optic sensors ; Gold compounds ; Metal nanoparticles ; Peptides ; Plasma stability
EI分类号	Medicine and Pharmacology:461.6 ; Biology:461.9 ; Fiber Optics:741.1.2 ; Nanotechnology:761 ; Organic Compounds:804.1 ; Plasma Physics:932.3
ESI学科分类	MATERIALS SCIENCE
来源库	EV Compendex
引用统计	被引频次[WOS]：4
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/677933
专题	工学院_生物医学工程系
作者单位	1.Engineering Laboratory of Peptides of Chinese Academy of Sciences, Key Laboratory of Bioactive Peptides of Yunnan Province, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, National Resource Center for Non-Human Primates, National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Sino-African Joint Research Center, New Cornerstone Science Institute, Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan, Kunming; 650223, China 2.Department of Zoology, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing; 210095, China 3.School of Molecular Medicine, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Zhejiang, Hangzhou; 310024, China 4.Department of Pharmaceutical Sciences, College of Pharmaceutical Sciences, Soochow University, Jiangsu, Suzhou; 215123, China 5.Department of Biomedical Engineering, Southern University of Science and Technology, Guangdong, Shenzhen; 518055, China 6.Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Zhejiang, Hangzhou; 310022, China 7.Department of Clinical Laboratory, First Affiliated Hospital of Kunming Medical College, Yunnan, Kunming; 650032, China 8.Kunming College of Life Science, University of Chinese Academy of Sciences, Yunnan, Kunming; 650204, China
推荐引用方式 GB/T 7714	Zhang, Zhiye,Chen, Yaoyao,Gao, Jinai,et al. Orientational Nanoconjugation with Gold Endows Marked Antimicrobial Potential and Drugability of Ultrashort Dipeptides[J]. Nano Letters,2023,23(24).
APA	Zhang, Zhiye.,Chen, Yaoyao.,Gao, Jinai.,Yang, Min.,Zhang, Dengdeng.,...&Lai, Ren.(2023).Orientational Nanoconjugation with Gold Endows Marked Antimicrobial Potential and Drugability of Ultrashort Dipeptides.Nano Letters,23(24).
MLA	Zhang, Zhiye,et al."Orientational Nanoconjugation with Gold Endows Marked Antimicrobial Potential and Drugability of Ultrashort Dipeptides".Nano Letters 23.24(2023).