Magnetic microspheres enhanced peanut structure cascaded lasso shaped fiber laser biosensor for cancer marker-CEACAM5 detection in serum

Hu，Jie1; He，Panpan2; Zhao，Fang1; Lin，Weihao1; Xue，Chenlong1; Chen，Jinna1; Yu，Zhiguang2; Ran，Yang3; Meng，Yue4; Hong，Xin5,6; Shum，Perry Ping1; Shao，Liyang1

2024-05-01

10.1016/j.talanta.2024.125625

Talanta   影响因子和分区

0039-9140

The detection of trace cancer markers in body fluids such as blood/serum is crucial for cancer diseases screening and treatment, which requires high sensitivity and specificity of biosensors. In this study, a peanut structure cascaded lasso (PSCL) shaped fiber sensing probe based on fiber laser demodulation method was proposed to specifically detect the carcinoembryonic antigen related cell adhesion molecules 5 (CEACAM5) protein in serum. Thanks for the narrow linewidth and high signal-to-noise ratio (SNR) of the laser spectrum, it is easier to distinguish small spectral changes than interference spectrum. Adding the antibody modified magnetic microspheres (MMS) to form the sandwich structure of "antibody-antigen-antibody-MMS", and amplified the response caused by biomolecular binding. The limit of detection (LOD) for CEACAM5 in buffer could reach 0.11 ng/mL. Considering the common threshold of 5 ng/mL for CEA during medical screening and the cut off limit of 2.5 ng/mL for some kits, the LOD of proposed biosensor meets the actual needs. Human serum samples from a hospital were used to validate the real sensing capability of proposed biosensor. The deviation between the measured value in various serum samples and the clinical value ranged from 1.9 to 9.8 %. This sensing scheme holds great potential to serve as a point of care testing (POCT) device and extend to more biosensing applications.

CEACAM5 protein Fiber laser Human serum Magnetic microsphere Optical fiber biosensor

SCI ; EI

英语

第一 ; 通讯

CHEMISTRY

2-s2.0-85182882470

Scopus

1.Department of Electronic and Electrical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
2.Medcaptain Medical Technology Co.,Ltd.,Shenzhen,518055,China
3.Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications,Institute of Photonics Technology,Jinan University,Guangzhou,510632,China
4.Department of Clinical Laboratory,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou,511436,China
5.Department of Biochemistry,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
6.Key University Laboratory of Metabolism and Health of Guangdong,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China

Hu，Jie,He，Panpan,Zhao，Fang,et al. Magnetic microspheres enhanced peanut structure cascaded lasso shaped fiber laser biosensor for cancer marker-CEACAM5 detection in serum[J]. Talanta,2024,271.

Hu，Jie.,He，Panpan.,Zhao，Fang.,Lin，Weihao.,Xue，Chenlong.,...&Shao，Liyang.(2024).Magnetic microspheres enhanced peanut structure cascaded lasso shaped fiber laser biosensor for cancer marker-CEACAM5 detection in serum.Talanta,271.

Hu，Jie,et al."Magnetic microspheres enhanced peanut structure cascaded lasso shaped fiber laser biosensor for cancer marker-CEACAM5 detection in serum".Talanta 271(2024).