Nanoparticles insert a three dimensional cavity structure of proteins for function inhibition: The Case of CeO2 and SARS-CoV-2

Zhang，Guofang1,2; Wang，Xiaofeng1,2; Wang，Guocheng3; Suo，Xiaoman1,2; Qiu，Yu1,2; Luo，Rong Hua4; Liu，Yingnan1,2,5; Li，Qi1,2; Luo，Wenhe1,2; Shi，Zhenzhen6; Liu，Fangfang1,2; Li，Zhongda1,2; Qu，Jing7; Li，Liang7; Bastús，Neus G.8,9; Himly，Martin5; Wang，Liming10; Liu，Feng Liang4; Zheng，Yong Tang4; Puntes，Victor9,11,12; Li，Yang1,2

2024-04-01

10.1016/j.nantod.2024.102183

Nano Today   影响因子和分区

1748-0132

1878-044X

The selective interaction of nanomaterials with proteins for protein function suppression has been reported. However, whether the nanomaterials could be used to target a three-dimensional (3D) structure of proteins for the consequent function inhibition is not defined. When SARS-CoV-2 binds to the host cell surface ACE2 receptor, the spike protein trimer changes to an “Open State” which forms a 5 nm cavity structure, consequently exposing the receptor binding domain (RBD) for the following viral infection. We found that the 3 nm cerium oxide nanoparticles (CeO@3) showed a better anti-SARS-CoV-2 effect than 30 nm cerium oxide nanoparticles (CeO@30). We performed a series of experiments and demonstrated that the CeO@3 could target the 5 nm spike protein trimer cavity and tightly bind with the RBD, thus effectively blocking the following virus-cell interaction and rendering CeO@3 as an effective anti-viral agent. As all coronaviruses possess similar spike protein structures as homologous proteins, CeO@3 can be used as a broad-sperm anti-coronavirus nanodrug candidate by targeting the spike protein 3D structure. This work, for the first time, demonstrated that rationally engineered inorganic nanomaterials can be used to specifically target a 3D structure of a certain protein for function inhibition, thus providing a novel methodological approach and paving the way for future molecular targeting nanodrug candidate design.

Cerium oxide nanoparticles Protein 3D Structure Protein corona SARS-CoV-2 Selective binding protein

SCI ; EI

英语

其他

2-s2.0-85184781071

Scopus

1.Laboratory of Inflammation and Vaccines,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong,518055,China
2.Laboratory of Immunology and Nanomedicine & China-Italy Joint Laboratory of Pharmacobiotechnology for Medical Immunomodulation,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong,518055,China
3.Research Center for Human Tissues & Organs Degeneration,Shenzhen Institute of Advanced Technology,Chinese Academic of Sciences,Shenzhen,Guangdong,518055,China
4.Key Laboratory of Bioactive Peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/,KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases,Center for Biosafety Mega-Science,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,Yunnan,650223,China
5.Dept. Biosciences & Medical Biology,Paris Lodron University of Salzburg,Salzburg,5020,Austria
6.The Program for Experimental and Theoretical Modeling,Division of Hepatology,Department of Medicine,Stritch School of Medicine,Loyola University Chicago,Maywood,60153,United States
7.Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
8.Instiut Català de Nanociència I Nanotecnologia (ICN2),CSIC and The Barcelona Institute of Science and Technology (BIST),Campus UAB,Barcelona,08193,Spain
9.CIBER en Bioingeniería,Biomateriales y Nanomedicina,CIBER-BBN,Spain; Vall d'Hebron Institut de Recerca (VHIR),Barcelona,08035,Spain
10.CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing,100049,China
11.Vall d'Hebron Institut de Recerca (VHIR),Barcelona,08035,Spain
12.Institució Catalana de Recerca i Estudis Avançats (ICREA),Barcelona,08010,Spain

Zhang，Guofang,Wang，Xiaofeng,Wang，Guocheng,et al. Nanoparticles insert a three dimensional cavity structure of proteins for function inhibition: The Case of CeO2 and SARS-CoV-2[J]. Nano Today,2024,55.

Zhang，Guofang.,Wang，Xiaofeng.,Wang，Guocheng.,Suo，Xiaoman.,Qiu，Yu.,...&Li，Yang.(2024).Nanoparticles insert a three dimensional cavity structure of proteins for function inhibition: The Case of CeO2 and SARS-CoV-2.Nano Today,55.

Zhang，Guofang,et al."Nanoparticles insert a three dimensional cavity structure of proteins for function inhibition: The Case of CeO2 and SARS-CoV-2".Nano Today 55(2024).