Investigating the antibiotic resistance genes and their potential risks in the megacity water environment: A case study of Shenzhen Bay Basin, China

Liang，Hebin1; Huang，Jin1; Tao，Yi1; Klümper，Uli2; Berendonk，Thomas U.2; Zhou，Kai3; Xia，Yu4; Yang，Ying5,6; Yu，Yang7,8; Yu，Ke9; Lin，Lin1; Li，Xiaoyan1; Li，Bing1

2024-03-05

10.1016/j.jhazmat.2024.133536

Journal of Hazardous Materials   影响因子和分区

0304-3894

1873-3336

Antibiotic resistance genes (ARGs) constitute emerging pollutants and pose serious risks to public health. Anthropogenic activities are recognized as the main driver of ARG dissemination in coastal regions. However, the distribution and dissemination of ARGs in Shenzhen Bay Basin, a typical megacity water environment, have been poorly investigated. Here, we comprehensively profiled ARGs in Shenzhen Bay Basin using metagenomic approaches, and estimated their associated health risks. ARG profiles varied greatly among different sampling locations with total abundance ranging from 2.79 × 10 (Shenzhen Bay sediment) to 1.04 (hospital sewage) copies per 16S rRNA gene copy, and 45.4% of them were located on plasmid-like sequences. Sewage treatment plants effluent and the corresponding tributary rivers were identified as the main sources of ARG contamination in Shenzhen Bay. Mobilizable plasmids and complete integrons carrying various ARGs probably participated in the dissemination of ARGs in Shenzhen Bay Basin. Additionally, 19 subtypes were assigned as high-risk ARGs (Rank I), and numerous ARGs were identified in potential human-associated pathogens, such as Burkholderiaceae, Rhodocyclaceae, Vibrionaceae, Pseudomonadaceae, and Aeromonadaceae. Overall, Shenzhen Bay represented a higher level of ARG risk than the ocean environment based on quantitative risk assessment. This study deepened our understanding of the ARGs and the associated risks in the megacity water environment.

Antibiotic resistance genes Megacity water environment Metagenomics Mobility Risk assessment

SCI

英语

其他

ENGINEERING

2-s2.0-85183781060

Scopus

1.State Environmental Protection Key Laboratory of Microorganism Application and Risk Control,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen,518055,China
2.Institute for Hydrobiology,Technische Universität Dresden,Dresden,01217,Germany
3.Shenzhen Institute of Respiratory Disease,Shenzhen People's Hospital (the First Affiliated Hospital,Southern University of Science and Technology; the Second Clinical Medical College,Jinan University),Shenzhen,518020,China
4.School of Environmental Science and Engineering,College of Engineering,Southern University of Science and Technology,Shenzhen,518055,China
5.School of Marine Sciences,Sun Yat-Sen University,Zhuhai,519082,China
6.Southern Marine Science and Engineering Guangdong Laboratory,Zhuhai,519000,China
7.National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria,South China Agricultural University,Guangzhou,510642,China
8.Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation,South China Agricultural University,Guangzhou,510642,China
9.School of Environment and Energy,Shenzhen Graduate School,Peking University,Shenzhen,518055,China

Liang，Hebin,Huang，Jin,Tao，Yi,et al. Investigating the antibiotic resistance genes and their potential risks in the megacity water environment: A case study of Shenzhen Bay Basin, China[J]. Journal of Hazardous Materials,2024,465.

Liang，Hebin.,Huang，Jin.,Tao，Yi.,Klümper，Uli.,Berendonk，Thomas U..,...&Li，Bing.(2024).Investigating the antibiotic resistance genes and their potential risks in the megacity water environment: A case study of Shenzhen Bay Basin, China.Journal of Hazardous Materials,465.

Liang，Hebin,et al."Investigating the antibiotic resistance genes and their potential risks in the megacity water environment: A case study of Shenzhen Bay Basin, China".Journal of Hazardous Materials 465(2024).