Self-lysis microbial consortia for predictable multi-proteins assembly

Zhang，Xi1; Li，Pengcheng1; Wang，Weijie1; Zhao，Wenjuan1; Dai，Shengkun1; Wang，Jie2; Li，Nan1; Dai，Zhuojun1

2024-03-01

10.1016/j.bioorg.2024.107117

Bioorganic Chemistry   影响因子和分区

0045-2068

1090-2120

The scope of bioengineering is expanding from the design of single strain to the microbial communities, allowing for the division-of-labor in synthesizing the multi-protein systems. Predicting the composition of the final product during the biomanufacturing process, however, can be difficult. Consortia-based manufacturing has the potential to boost production efficiency, but this benefit primarily holds in the upstream. The current format of downstream process heavily relies on the centralized facility, and is not economical and flexible to address the demands in small-scale. Here, we present a concise and manageable platform to enable the multi-protein system assembly. We engineer a self-lysis microbial consortium, where each strain lyses autonomously at high densities and produces a single protein component. The product fraction can be precisely tuned by varying the inoculation ratio. Utilizing this platform, we assemble a classical 34-component PURE (protein synthesis using recombinant elements) system. We have further optimized the downstream process of the biomanufacturing by incorporating the porous structure of polymeric materials. The encapsulated autolysis consortium can produce and release the proteins while maintaining the cell factories enclosed in the materials by exporting the multi-protein system for collection. Our research provides a novel approach to the flexible and controllable production of multi-protein systems, opening up new possibilities for pathway assembly and portable biomanufacturing.

SCI

英语

其他

BIOLOGY & BIOCHEMISTRY

2-s2.0-85183631000

Scopus

1.Key Laboratory of Quantitative Synthetic Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China
2.Department of Chemistry,Research Center for Chemical Biology and Omics Analysis,College of Science,Guangdong Provincial Key Laboratory of Catalysis,Southern University of Science and Technology,Shenzhen,518055,China

Zhang，Xi,Li，Pengcheng,Wang，Weijie,et al. Self-lysis microbial consortia for predictable multi-proteins assembly[J]. Bioorganic Chemistry,2024,144.

Zhang，Xi.,Li，Pengcheng.,Wang，Weijie.,Zhao，Wenjuan.,Dai，Shengkun.,...&Dai，Zhuojun.(2024).Self-lysis microbial consortia for predictable multi-proteins assembly.Bioorganic Chemistry,144.

Zhang，Xi,et al."Self-lysis microbial consortia for predictable multi-proteins assembly".Bioorganic Chemistry 144(2024).