The pleiotropic enhancer enh9 promotes cell proliferation and migration in non-small cell lung cancer via ERMP1 and PD-L1

Wang，Qilin1,2,3; Zhang，Junyou1,2,3; Wen，Yanling4,5; Qi，Sihan1,2,3; Duan，Yingying1,2,3; Liu，Qian1,2,3; Li，Chunyan1,2,3,6

2024-03-01

10.1016/j.bbadis.2023.167015

Biochimica et Biophysica Acta - Molecular Basis of Disease   影响因子和分区

0925-4439

1879-260X

Enhancers, cis-acting DNA elements for transcriptional regulation, are important regulators of cell identity and disease. However, of the hundreds of thousands of enhancers annotated in the human genome, only a few have been studied for their regulatory mechanisms and functions in cancer progression and therapeutic resistance. Here, we report the pleiotropy of one enhancer (named enh9) in both cell proliferation and migration in non-small cell lung cancer (NSCLC) cells. By integrating multi-genomic data, ERMP1 and PD-L1 were screened out as potential targets of enh9. CUT&Tag sequencing demonstrated that enh9 was involved in the genomic interactions between the transcription factor RELA and the promoters of ERMP1 and PD-L1. In addition, ERMP1 and PD-L1 were validated to be involved in cell proliferation and migration, respectively. Our study fully elucidated the function and transcriptional regulation mechanisms of enh9 in NSCLC. The exploration on enhancers is promising to provide new insights for cancer diagnosis and therapy.

Enhancer Migration Non-small cell lung cancer Pleiotropy Proliferation

SCI

英语

其他

CLINICAL MEDICINE

2-s2.0-85182665953

Scopus

1.School of Engineering Medicine,Beihang University,Beijing,100191,China
2.Key Laboratory of Big Data-Based Precision Medicine (Ministry of Industry and Information Technology),Beihang University,Beijing,100191,China
3.School of Biological Science and Medical Engineering,Beihang University,Beijing,100191,China
4.Institute for Hepatology,National Clinical Research Center for Infectious Disease,Shenzhen Third People's Hospital,Shenzhen,Guangdong,518112,China
5.The Second Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,518112,China
6.Beijing Advanced Innovation Center for Big Data-Based Precision Medicine,Beihang University,Beijing,100191,China

Wang，Qilin,Zhang，Junyou,Wen，Yanling,et al. The pleiotropic enhancer enh9 promotes cell proliferation and migration in non-small cell lung cancer via ERMP1 and PD-L1[J]. Biochimica et Biophysica Acta - Molecular Basis of Disease,2024,1870(3).

Wang，Qilin.,Zhang，Junyou.,Wen，Yanling.,Qi，Sihan.,Duan，Yingying.,...&Li，Chunyan.(2024).The pleiotropic enhancer enh9 promotes cell proliferation and migration in non-small cell lung cancer via ERMP1 and PD-L1.Biochimica et Biophysica Acta - Molecular Basis of Disease,1870(3).

Wang，Qilin,et al."The pleiotropic enhancer enh9 promotes cell proliferation and migration in non-small cell lung cancer via ERMP1 and PD-L1".Biochimica et Biophysica Acta - Molecular Basis of Disease 1870.3(2024).