Optical Resolution Photoacoustic Microscopy Reveals VEGF-induced Angiogenesis During Bone Regeneration

Chen, Qian1,2; Wang, Ziyan3; Xie, Huikai1,2,4

10.1117/12.2683773

2023

Conference on Optics in Health Care and Biomedical Optics XIII

0277-786X

1996-756X

978-1-5106-6789-1

OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS XIII

12770

OCT 14-16, 2023

null,Beijing,PEOPLES R CHINA

1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

SPIE-INT SOC OPTICAL ENGINEERING

Angiogenesis is essential for bone homeostasis and repair. Newly formed vessels convey osteogenic progenitors during bone regeneration. However, the lack of continuous and label-free visualization of the bone microvasculature has resulted in little understanding of the neovascular dynamics. Here, we take advantage of optical-resolution photoacoustic microscopy (ORPAM) for label-free, intravital, long-term observation of the bone vascular dynamics. We quantify the angiogenic effect of locally-applied vascular endothelial growth factor (VEGF) in the mouse tibia defect model. VEGF treatment increased the concentration of total hemoglobin, vascular branching, and vascular density, which increased bone formation within the defect. These data demonstrated ORPAM as a useful imaging tool understand bone angiogenesis, and revealed the effectiveness of locally delivered therapeutic agents with sufficient sensitivity, contributing to the studying the bone regeneration in the future.

Bone regeneration angiogenesis VEGF ORPAM

其他

英语

EI ; CPCI-S

Biochemistry & Molecular Biology ; Engineering ; Optics ; Radiology, Nuclear Medicine & Medical Imaging

Biochemical Research Methods ; Engineering, Biomedical ; Optics ; Radiology, Nuclear Medicine & Medical Imaging

WOS:001150986900018

Web of Science

1.BIT Chongqing Institute of Microelectronics and Microsystems, Chongqing; 400000, China
2.School of Integrated Circuits and Electronics, Beijing Institute of Technology, Beijing; 100081, China
3.Department of Biomedical Engineering, Southern University of Science and Technology, Guangdong, Shenzhen; 518055, China
4.LightVision Technologies Inc. Ltd., Foshan; 528000, China

Chen, Qian,Wang, Ziyan,Xie, Huikai. Optical Resolution Photoacoustic Microscopy Reveals VEGF-induced Angiogenesis During Bone Regeneration[C]. 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA:SPIE-INT SOC OPTICAL ENGINEERING,2023.