Transient anoxic conditions boost N2O emissions by stimulating denitrification capacity and decreasing N2O reduction ratio in soils with different carbon substrates

Zhuang，Shan1,2; Ding，Junjun1,3,8; Lin，Wei4; Zheng，Qian5; Kou，Xinyue1; Li，Qiaozhen1,3; Xu，Chunying1,3; Mao，Lili1,3; Pan，Yanshuo1; Gao，Ying6,7; Han，Dongfei2,8; Li，Yuzhong1,3

2024-05-01

10.1016/j.soilbio.2024.109351

Soil Biology and Biochemistry   影响因子和分区

0038-0717

Quantitative identification of the dominant NO production and consumption processes following sudden shifts in soil aeration in well-aerated soils is limited, lagging the development of NO mitigation strategies. Nitrous oxide emissions from agricultural soils, the largest source of anthropogenic NO, are strongly influenced by low-molecular-weight organic carbons from root exudates (RootEx). In this study, we conducted a laboratory incubation experiment to investigate the mechanisms of NO emissions regulated by transient anoxic conditions under the effects of different carbon substrates. Three common components in RootEx, oxalic acid, serine, and glucose were individually added as carbon sources every 24 h to the sandy loam soil. The contribution of microbial processes to NO production (fungal denitrification, nitrification, bacterial denitrification) and consumption (NO reduction) was evaluated using a dual isotope mapping approach (SP/δO MAP). The result indicated that transient anoxic conditions stimulated NO production primarily through bacterial denitrification process facilitated by carbon substrates, and diminished the proportion of NO reduction compared to oxic conditions. Among the three components of RootEx, serine and glucose induced higher NO emissions and NO reduction rates than oxalic acid during incubation. The differential effects of these three components on NO emissions imply that the regulatory effect of carbon substrates on NO reduction could not counterbalance the influence of transient anoxic conditions on NO stimulation. Our findings provide a fundamental understanding of the effects of transient anoxic conditions and RootEx on NO stimulation, facilitating the study of the spatial and temporal heterogeneity of NO emissions in response to soil aeration changes in various upland soils.

Dual isotope mapping Glucose N2O reduction Nitrous oxide Oxalic acid Serine Transient anoxic conditions

SCI ; EI

英语

其他

AGRICULTURAL SCIENCES

2-s2.0-85185887143

Scopus

1.Institute of Environment and Sustainable Development in Agriculture,Chinese Academy of Agricultural Sciences,Beijing,100081,China
2.School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou,215009,China
3.Environmental Stable Isotope Lab,Chinese Academy of Agricultural Sciences,Beijing,100081,China
4.Institute of Urban Agriculture,Chinese Academy of Agricultural Sciences,Chengdu,610213,China
5.Department of Ocean Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
6.Institute of Ecological Conservation and Restoration,Chinese Academy of Forestry,Beijing,100091,China
7.Institute of Desertification Studies,Chinese Academy of Forestry,Beijing,100091,China
8.State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China,Beijing,100081,China

Zhuang，Shan,Ding，Junjun,Lin，Wei,et al. Transient anoxic conditions boost N2O emissions by stimulating denitrification capacity and decreasing N2O reduction ratio in soils with different carbon substrates[J]. Soil Biology and Biochemistry,2024,192.

Zhuang，Shan.,Ding，Junjun.,Lin，Wei.,Zheng，Qian.,Kou，Xinyue.,...&Li，Yuzhong.(2024).Transient anoxic conditions boost N2O emissions by stimulating denitrification capacity and decreasing N2O reduction ratio in soils with different carbon substrates.Soil Biology and Biochemistry,192.

Zhuang，Shan,et al."Transient anoxic conditions boost N2O emissions by stimulating denitrification capacity and decreasing N2O reduction ratio in soils with different carbon substrates".Soil Biology and Biochemistry 192(2024).