南方科技大学知识苑(SUSTech KC): Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9

题名	Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9
作者	Sun，Yonghua 1; Zhang，Bo 2; Luo，Lingfei 3; Shi，De Li 4; Wang，Han 5; Cui，Zongbin 1; Huang，Honghui 3; Cao，Ying 6; Shu，Xiaodong 7; Zhang，Wenqing 8; Zhou，Jianfeng 9; Li，Yun 9; Du，Jiulin 10; Zhao，Qingshun 11; Chen，Jun 12; Zhong，Hanbing13 ; Zhong，Tao P.14; Li，Li 3; Xiong，Jing Wei 15; Peng，Jinrong 12; Xiao，Wuhan 1; Zhang，Jian 16; Yao，Jihua 17; Yin，Zhan 1; Mo，Xianming 18; Peng，Gang 19; Zhu，Jun 20; Chen，Yan 21; Zhou，Yong 22; Liu，Dong13 ; Pan，Weijun 22; Zhang，Yiyue 8; Ruan，Hua 3; Liu，Feng 23; Zhu，Zuoyan 1; Meng，Anming 24
通讯作者	Liu，Feng
发表日期	2020
DOI	10.1101/gr.248559.119
发表期刊	GENOME RESEARCH 影响因子和分区
ISSN	1088-9051
EISSN	1549-5469
卷号	30 期号:1页码:118-126
摘要	Genome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism has rarely been achieved. Here, we performed a systematic knockout of all the genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, by high-throughput bioinformatics analysis, we found that sequence features play pivotal roles in effective gRNA targeting at specific genes of interest, while the success rate of gene targeting positively correlates with GC content of the target sites. Moreover, we found that nearly one-fourth of all mutants are related to human diseases, and several representative CRISPR/Cas9-generated mutants are described here. Furthermore, we tried to identify the underlying mechanisms leading to distinct phenotypes between genetic mutants and antisense morpholino-mediated knockdown embryos. Altogether, this work has generated the first chromosome-wide collection of zebrafish genetic mutants by the CRISPR/Cas9 technology, which will serve as a valuable resource for the community, and our bioinformatics analysis also provides some useful guidance to design gene-specific gRNAs for successful gene editing.
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
重要成果	NI论文
学校署名	其他
资助项目	National Natural Science Foundation of China[31830061] ; National Natural Science Foundation of China[31721005]
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000506573000011
出版者	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
ESI学科分类	MOLECULAR BIOLOGY & GENETICS
Scopus记录号	2-s2.0-85078099434
来源库	Scopus
引用统计	被引频次[WOS]：30
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/73644
专题	生命科学学院_生物系生命科学学院
作者单位	1.State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Innovation Academy for Seed Design,Chinese Academy of Sciences,Wuhan, Hubei,430072,China 2.Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education,Peking University Genome Editing Research Center,College of Life Sciences,Peking University,Beijing,100871,China 3.School of Life Sciences,Southwest University,Chongqing,400715,China 4.Guangdong Medical University,Zhanjiang, Guangdong,524023,China 5.Center for Circadian Clocks,Soochow University,Suzhou,215123,China 6.School of Life Sciences and Technology,Tongji University,Shanghai,200092,China 7.Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou, Guangdong,510530,China 8.Division of Cell,Developmental and Integrative Biology,School of Medicine,South China University of Technology,Guangzhou,510006,China 9.School of Medicine and Pharmacy,Ocean University of China,Qingdao, Shandong,266100,China 10.State Key Laboratory of Neuroscience,Institute of Neuroscience,Chinese Academy of Sciences,Shanghai,200031,China 11.Model Animal Research Center,Nanjing University,Nanjing, Jiangsu,210093,China 12.College of Life Sciences,Zhejiang University,Hangzhou,310058,China 13.Department of Biology,South University of Science and Technology of China,Shenzhen, Guangdong,518055,China 14.Institute of Biomedical Sciences,East China Normal University,Shanghai,200062,China 15.College of Life Sciences,Institute of Molecular Medicine,Peking University,Beijing,100871,China 16.School of Life Sciences,Yunnan University,Kunming,650091,China 17.School of Life Sciences,Fudan University,Shanghai,200433,China 18.State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu,610041,China 19.Institutes of Brain Science,Fudan University,Shanghai,200433,China 20.Sino-French Research Center for Life Sciences and Genomics,Rui-Jin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200025,China 21.Institute of Health Sciences,Chinese Academy of Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai,200025,China 22.CAS Key Laboratory of Nutrition,Metabolism and Food Safety,Shanghai Institute of Nutrition and Health,Chinese Academy of Sciences,Shanghai,200031,China 23.State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing,100101,China 24.School of Life Sciences,Tsinghua University,Beijing,100084,China
推荐引用方式 GB/T 7714	Sun，Yonghua,Zhang，Bo,Luo，Lingfei,et al. Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9[J]. GENOME RESEARCH,2020,30(1):118-126.
APA	Sun，Yonghua.,Zhang，Bo.,Luo，Lingfei.,Shi，De Li.,Wang，Han.,...&Meng，Anming.(2020).Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9.GENOME RESEARCH,30(1),118-126.
MLA	Sun，Yonghua,et al."Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9".GENOME RESEARCH 30.1(2020):118-126.

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