The development of a novel zeolite-based assay for efficient and deep plasma proteomic profiling

Li，Nan1; Huang，Jingnan2,3; He，Shangwen8; Zheng，Qiaocong1,4; Ye，Feng1; Qin，Zhengxing5; Wang，Dong6; Xiao，Ting1; Mao，Mengyuan1; Zhou，Zhenhua1; Tang，Tingxi1; Zhang，Longshan1; Wang，Xiaoqing1; Wang，Yingqiao1; Lyu，Ying7; Liu，Laiyu8; Dai，Lingyun2,3; Wang，Jigang2,3; Guan，Jian1,9

2024-12-01

10.1186/s12951-024-02404-9

Journal of Nanobiotechnology   影响因子和分区

1477-3155

Plasma proteins are considered the most informative source of biomarkers for disease diagnosis and monitoring. Mass spectrometry (MS)-based proteomics has been applied to identify biomarkers in plasma, but the complexity of the plasma proteome and the extremely large dynamic range of protein abundances in plasma make the clinical application of plasma proteomics highly challenging. We designed and synthesized zeolite-based nanoparticles to deplete high-abundance plasma proteins. The resulting novel plasma proteomic assay can measure approximately 3000 plasma proteins in a 45 min chromatographic gradient. Compared to those in neat and depleted plasma, the plasma proteins identified by our assay exhibited distinct biological profiles, as validated in several public datasets. A pilot investigation of the proteomic profile of a hepatocellular carcinoma (HCC) cohort identified 15 promising protein features, highlighting the diagnostic value of the plasma proteome in distinguishing individuals with and without HCC. Furthermore, this assay can be easily integrated with all current downstream protein profiling methods and potentially extended to other biofluids. In conclusion, we established a robust and efficient plasma proteomic assay with unprecedented identification depth, paving the way for the translation of plasma proteomics into clinical applications. Graphical Abstract: (Figure presented.)

Plasma Protein corona Proteomics Zeolite

SCI

英语

其他

BIOLOGY & BIOCHEMISTRY

2-s2.0-85190156451

Scopus

1.Department of Radiation Oncology,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong,510515,China
2.Department of Nephrology,Shenzhen Key Laboratory of Kidney Diseases,Shenzhen People’s Hospital,(The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,Guangdong,518020,China
3.School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China
4.Department of Oncology,People’s Hospital of YangJiang,Yangjiang,Guangdong,529500,China
5.State Key Laboratory of Heavy Oil Processing,College of Chemical Engineering,China University of Petroleum (East China),Qingdao,Shandong,266580,China
6.College of Chemistry and Chemical Engineering,China University of Petroleum (East China),Qingdao,Shandong,266580,China
7.Department of Traditional Chinese Medicine,Nanfang Hospital,,Southern Medical University,Guangzhou,Guangdong,510515,China
8.Chronic Airways Diseases Laboratory,Department of Respiratory and Critical Care Medicine,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong,510515,China
9.Guangdong Province Key Laboratory of Molecular Tumor Pathology,Guangzhou,Guangdong,510515,China

Li，Nan,Huang，Jingnan,He，Shangwen,et al. The development of a novel zeolite-based assay for efficient and deep plasma proteomic profiling[J]. Journal of Nanobiotechnology,2024,22(1).

Li，Nan.,Huang，Jingnan.,He，Shangwen.,Zheng，Qiaocong.,Ye，Feng.,...&Guan，Jian.(2024).The development of a novel zeolite-based assay for efficient and deep plasma proteomic profiling.Journal of Nanobiotechnology,22(1).

Li，Nan,et al."The development of a novel zeolite-based assay for efficient and deep plasma proteomic profiling".Journal of Nanobiotechnology 22.1(2024).