An array of femtoliter wells for sensitive detection of copper using click chemistry

Ge，Chenchen1; Chen，Xiong2; Wang，Dou3

2024-07-01

10.1016/j.talanta.2024.125973

Talanta   影响因子和分区

0039-9140

Sensitive detection of copper ion (Cu), which is of great importance for environmental pollution and human health, is crucial. In this study, we present a highly sensitive method for measuring Cu in an array of femtoliter wells. In brief, magnetic beads (MBs) modified with alkyne groups were bound to the azide groups of biotin-PEG3-azide (bio-PEG-N) via Cu-catalyzed click chemistry. Cu in the click chemistry reaction was generated by reducing Cu with sodium ascorbate. Following the ligation, the surface of the MBs was modified with biotin, which could be labeled with streptavidin-β-galactosidase (SβG). The MBs complex was then suspended in β-galactosidase substrate fluorescein-di-β-D-galactopyranoside (FDG), and loaded into the array of femtoliter wells. The MBs sank into the wells due to gravity, and the resulting fluorescent product, generated from the reaction between SβG on the surface of the MBs and FDG, was confined within the wells. The number of fluorescent wells increased with higher Cu concentrations. The bright-field and fluorescent images of the wells were acquired using an inverted fluorescent microscope. The detection limit of this assay for Cu was 1 nM without signal amplification, which was 10 times lower than that of traditional fluorescence detection assays.

Click chemistry Cu2+ detection Femtoliter wells Fluorescence detection Microfluidic chip

SCI ; EI

英语

通讯

20241315823950

Coenzymes ; Copper ; Fluorescence ; Metal ions ; Synthesis (chemical)

Metallurgy:531.1 ; Copper:544.1 ; Microfluidics:632.5.1 ; Light/Optics:741.1 ; Chemical Reactions:802.2

CHEMISTRY

2-s2.0-85188683967

Scopus

1.College of Health Science and Environmental Engineering,Shenzhen Technology University,Shenzhen,3002 Lantian Road, Pingshan District, Guangdong,518118,China
2.The Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,School of Public Health,Guizhou Medical University,Guiyang,Guizhou,550025,China
3.Shenzhen Key Laboratory of Smart Healthcare Engineering,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,No. 1088, Xueyuan Rd., Xili, Nanshan District, Guangdong,518055,China

Ge，Chenchen,Chen，Xiong,Wang，Dou. An array of femtoliter wells for sensitive detection of copper using click chemistry[J]. Talanta,2024,274.

Ge，Chenchen,Chen，Xiong,&Wang，Dou.(2024).An array of femtoliter wells for sensitive detection of copper using click chemistry.Talanta,274.

Ge，Chenchen,et al."An array of femtoliter wells for sensitive detection of copper using click chemistry".Talanta 274(2024).