南方科技大学知识苑(SUSTech KC): Ultra-highly selective recognition of nucleosides over nucleotides by rational modification of tetralactam macrocycle and its application in enzyme assay

题名	Ultra-highly selective recognition of nucleosides over nucleotides by rational modification of tetralactam macrocycle and its application in enzyme assay
作者	Yao，Huan 1; Qin，Jian 1; Wang，Yan Fang2 ; Wang，Song Meng 2 ; Yi，Liu Huan 1; Li，Shi Yao 1; Du，Fangfang 1; Yang，Liu Pan 1; Wang，Li Li 1
发表日期	2024-06-01
DOI	10.1016/j.cclet.2023.109154
发表期刊	Chinese Chemical Letters 影响因子和分区
ISSN	1001-8417
卷号	35 期号:6
摘要	Artificial macrocycle with high binding selectivity in water is often challenging but urgently needed in various research and application areas. Herein, we report a new water-soluble biomimetic tetralactam macrocycle and realize the ultra-high selectivity to nucleosides over corresponding monophosphate nucleotides by rational modification. The introduction of charged groups at the periphery of endo-functionalized cavity makes the selectivity (guanosine to guanosine 5′-monophosphate) increase remarkably from 100 to 1119. Based on the ultra-high selectivity of biomimetic tetralactam macrocycle, the sensitive CD73 enzyme activity assay was then achieved through product-selective fluorescence indicator displacement assay. Furthermore, the capability of the proposed method for inhibitor screening was successfully displayed.
关键词	Biomimetic macrocycle Enzyme assay Rational modification Ultra-high selectivity
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	其他
ESI学科分类	CHEMISTRY
Scopus记录号	2-s2.0-85188005571
来源库	Scopus
引用统计	被引频次[WOS]：8
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/741123
专题	理学院_化学系
作者单位	1.School of Pharmaceutical Science,Postdoctoral Research Station of Basic Medicine,Hengyang Medical School,University of South China,Hengyang,421001,China 2.Department of Chemistry,Southern University of Science and Technology,Shenzhen,518055,China
推荐引用方式 GB/T 7714	Yao，Huan,Qin，Jian,Wang，Yan Fang,et al. Ultra-highly selective recognition of nucleosides over nucleotides by rational modification of tetralactam macrocycle and its application in enzyme assay[J]. Chinese Chemical Letters,2024,35(6).
APA	Yao，Huan.,Qin，Jian.,Wang，Yan Fang.,Wang，Song Meng.,Yi，Liu Huan.,...&Wang，Li Li.(2024).Ultra-highly selective recognition of nucleosides over nucleotides by rational modification of tetralactam macrocycle and its application in enzyme assay.Chinese Chemical Letters,35(6).
MLA	Yao，Huan,et al."Ultra-highly selective recognition of nucleosides over nucleotides by rational modification of tetralactam macrocycle and its application in enzyme assay".Chinese Chemical Letters 35.6(2024).