Single-cell transcriptome profiling reveals cell type-specific variation and development in HLA expression of human skin

Lin，Yumiao1; Li，Xinxin1; Fang，Jingxian2; Zeng，Qinglan1; Cheng，Danling3; Wang，Gaofeng4; Shi，Runlu5; Luo，Yilin1; Ma，Yihe1; Li，Miaomiao6; Tang，Xiang7; Wang，Xusheng1; Tian，Ruiyun8,9

2024-05-30

10.1016/j.intimp.2024.112070

International Immunopharmacology   影响因子和分区

1567-5769

1878-1705

Skin, the largest organ of body, is a highly immunogenic tissue with a diverse collection of immune cells. Highly polymorphic human leukocyte antigen (HLA) molecules have a central role in coordinating immune responses as recognition molecules. Nevertheless, HLA gene expression patterns among diverse cell types within a specific organ, like the skin, have yet to be thoroughly investigated, with stromal cells attracting much less attention than immune cells. To illustrate HLA expression profiles across different cell types in the skin, we performed single-cell RNA sequencing (scRNA-seq) analyses on skin datasets, covering adult and fetal skin, and hair follicles as the skin appendages. We revealed the variation in HLA expression between different skin populations by examining normal adult skin datasets. Moreover, we evaluated the potential immunogenicity of multiple skin populations based on the expression of classical HLA class I genes, which were well represented in all cell types. Furthermore, we generated scRNA-seq data of developing skin from fetuses of 15 post conception weeks (PCW), 17 PCW, and 22 PCW, delineating the dynamic expression of HLA genes with cell type-dependent variation among various cell types during development. Notably, the pseudotime trajectory analysis unraveled the significant variance in HLA genes during the evolution of vascular endothelial cells. Moreover, we uncovered the immune-privileged properties of hair follicles at single-cell resolution. Our study presents a comprehensive single-cell transcriptomic landscape of HLA genes in the skin, which provides new insights into variation in HLA molecules and offers a clue for allogeneic skin transplantation.

Hair follicle HLA genes Immune privilege Immunogenicity Single–cell sequencing Skin

SCI

英语

其他

PHARMACOLOGY & TOXICOLOGY

2-s2.0-85190737639

Scopus

1.School of Pharmaceutical Sciences (Shenzhen),Shenzhen Campus of Sun Yat-sen University,Sun Yat-sen University,Shenzhen,518107,China
2.Department of Pediatric Dentistry,Stomatological Hospital,Southern Medical University,Guangzhou,510280,China
3.Longgang District Maternity & Child Healthcare Hospital of Shenzhen City,Shenzhen,518172,China
4.Department of Pastic and Aesthetic Surgery,Nanfang Hospital of Southern Medical University,Guangzhou,510515,China
5.Tsinghua Shenzhen International Graduate School,Shenzhen,518055,China
6.Department of Hemangioma and Vascular Malformation Surgery,People's Hospital of Zhengzhou University,Zhengzhou,450003,China
7.Department of Minimal Invasive Gynecology,Guangzhou Women and Children's Hospital,Guangzhou Medical University,Guangzhou,510000,China
8.The First Affiliated Hospital (Shenzhen People's Hospital),Southern University of Science and Technology,Shenzhen,518055,China
9.GuangDong Engineering Technology Research Center of Stem Cell and Cell therapy,Shenzhen Key Laboratory of Stem Cell Research and Clinical Transformation,Shenzhen Immune Cell Therapy Public Service Platform,Shenzhen,518020,China

Lin，Yumiao,Li，Xinxin,Fang，Jingxian,et al. Single-cell transcriptome profiling reveals cell type-specific variation and development in HLA expression of human skin[J]. International Immunopharmacology,2024,133.

Lin，Yumiao.,Li，Xinxin.,Fang，Jingxian.,Zeng，Qinglan.,Cheng，Danling.,...&Tian，Ruiyun.(2024).Single-cell transcriptome profiling reveals cell type-specific variation and development in HLA expression of human skin.International Immunopharmacology,133.

Lin，Yumiao,et al."Single-cell transcriptome profiling reveals cell type-specific variation and development in HLA expression of human skin".International Immunopharmacology 133(2024).