Adolescent male rats show altered gut microbiota composition associated with depressive-like behavior after chronic unpredictable mild stress: Differences from adult rats

Wang，Jie1,2; Fan，Li2,3; Teng，Teng1,2; Wu，Hongyan1,2; Liu，Xueer1,2; Yin，Bangmin1,2; Li，Xuemei1,2; Jiang，Yuanliang1,2; Zhao，Jianting4; Wu，Qingyuan5; Guo，Yi6; Zhou，Xinyu1,2; Xie，Peng2,3

2024-05-01

10.1016/j.jpsychires.2024.03.026

Journal of Psychiatric Research   影响因子和分区

0022-3956

1879-1379

Accumulating evidence reveals the metabolism and neurotransmitter systems are different in major depressive disorder (MDD) between adolescent and adult patients; however, much is still unknown from the gut microbiome perspective. To minimize confounding factors such as geographical location, ethnicity, diet, and drugs, we investigated the gut microbial differences between adolescent and adult male Sprague-Dawley rats. We exposed the adolescent rats to chronic unpredictable mild stress (CUMS) for 3 weeks and assessed their behavior using the sucrose preference test (SPT), open field test (OFT), and forced swimming test (FST). We collected and sequenced fecal samples after the behavioral tests and compared them with our previous data on adult rats. Both adolescent and adult CUMS rats exhibited reduced sucrose preference in SPT, reduced total distance in OFT, and increased immobility time in FST. Moreover, compared to their respective controls, the adolescent CUMS rats had distinct amplicon sequence variants (ASVs) mainly in the Muribaculaceae family, Bacteroidetes phylum, while the adult CUMS rats had those in the Lachnospiraceae family, Firmicutes phylum. In the adolescent group, the Muribaculaceae negatively correlated with FST and positively correlated with SPT and OFT. In the adult group, the different genera in the Lachnospiraceae showed opposite correlations with FST. Furthermore, the adolescent CUMS rats showed disrupted microbial functions, such as “Xenobiotics biodegradation and metabolism” and “Immune system”, while the adult CUMS rats did not. These results confirmed the gut microbiota differences between adolescent and adult rats after CUMS modeling and provided new insight into the age-related influence on depression models.

Adolescent Adult Chronic unpredictable mild stress Depression Gut microbiota

SCI ; SSCI

英语

其他

PSYCHIATRY/PSYCHOLOGY

2-s2.0-85188932259

Scopus

1.Department of Psychiatry,The First Affiliated Hospital of Chongqing Medical University,Chongqing,China
2.NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases,The First Affiliated Hospital of Chongqing Medical University,Chongqing,China
3.Department of Neurology,The First Affiliated Hospital of Chongqing Medical University,Chongqing,China
4.Department of Neurology,Xinxiang Central Hospital,The Fourth Clinical College of Xinxiang Medical College,Xinxiang,China
5.Department of Neurology,Chongqing University Three Gorges Hospital,Chongqing,China
6.Department of Neurology,Shenzhen People's Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,Guangdong,China

Wang，Jie,Fan，Li,Teng，Teng,et al. Adolescent male rats show altered gut microbiota composition associated with depressive-like behavior after chronic unpredictable mild stress: Differences from adult rats[J]. Journal of Psychiatric Research,2024,173:183-191.

Wang，Jie.,Fan，Li.,Teng，Teng.,Wu，Hongyan.,Liu，Xueer.,...&Xie，Peng.(2024).Adolescent male rats show altered gut microbiota composition associated with depressive-like behavior after chronic unpredictable mild stress: Differences from adult rats.Journal of Psychiatric Research,173,183-191.

Wang，Jie,et al."Adolescent male rats show altered gut microbiota composition associated with depressive-like behavior after chronic unpredictable mild stress: Differences from adult rats".Journal of Psychiatric Research 173(2024):183-191.