Organic Fluorophores with Large Stokes Shift for the Visualization of Rapid Protein and Nucleic Acid Assays

Yang，Jingkai1; Xu，Ziyi1; Yu，Le3; Wang，Bingyun1; Hu，Ruibin1; Tang，Jiahu1; Lv，Jiahui1; Xiao，Hongjun1; Tan，Xuan1; Wang，Guanghui1; Li，Jia Xin2; Liu，Ying1; Shao，Pan Lin2; Zhang，Bo1

2024-04-22

10.1002/anie.202318800

Angewandte Chemie - International Edition   影响因子和分区

1433-7851

1521-3773

Organic small-molecule fluorophores, characterized by flexible chemical structure and adjustable optical performance, have shown tremendous potential in biosensing. However, classical organic fluorophore motifs feature large overlap between excitation and emission spectra, leading to the requirement of advanced optical set up to filter desired signal, which limits their application in scenarios with simple settings. Here, a series of wavelength-tunable small-molecule fluorescent dyes (PTs) bearing simple organic moieties have been developed, which exhibit Stokes shift up to 262 nm, molar extinction coefficients ranged 30,000–100,000 M cm, with quantum yields up to 54.8 %. Furthermore, these dyes were formulated into fluorescent nanoparticles (PT-NPs), and applied in lateral flow assay (LFA). Consequently, limit of detection for SARS-CoV-2 nucleocapsid protein reached 20 fM with naked eye, a 100-fold improvement in sensitivity compared to the pM detection level for colloidal gold-based LFA. Besides, combined with loop-mediated isothermal amplification (LAMP), the LFA system achieved the visualization of single copy level nucleic acid detection for monkeypox (Mpox).

Fluorescent nanoparticle Large stokes shift lateral flow assay Organic Fluorophores

SCI ; EI

英语

第一 ; 通讯

CHEMISTRY

2-s2.0-85188241100

Scopus

1.Guangdong Provincial Key Laboratory of Advanced Biomaterials,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,1088 Xueyuan Road,518055,China
2.Key Laboratory of Molecular Target & Clinical Pharmacology and the State Key Laboratory of Respiratory Disease,School of Pharmaceutical Sciences & the Fifth Affiliated Hospital,Guangzhou Medical University,Guangzhou,511436,China
3.Key Laboratory of Synthetic and Nature Molecule Chemistry of Ministry of Education,Department of Chemistry & Materials Science,Northwest University. Xi'an,Xi An Shi,710127,China

Yang，Jingkai,Xu，Ziyi,Yu，Le,et al. Organic Fluorophores with Large Stokes Shift for the Visualization of Rapid Protein and Nucleic Acid Assays[J]. Angewandte Chemie - International Edition,2024,63(17).

Yang，Jingkai.,Xu，Ziyi.,Yu，Le.,Wang，Bingyun.,Hu，Ruibin.,...&Zhang，Bo.(2024).Organic Fluorophores with Large Stokes Shift for the Visualization of Rapid Protein and Nucleic Acid Assays.Angewandte Chemie - International Edition,63(17).

Yang，Jingkai,et al."Organic Fluorophores with Large Stokes Shift for the Visualization of Rapid Protein and Nucleic Acid Assays".Angewandte Chemie - International Edition 63.17(2024).