Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays Bhartiya A.

Bhartiya，A.1,2; Madi，K.3; Disney，C. M.4; Courtois，L.3; Jupe，A.5; Zhang，F.1,2,6; Bodey，A. J.7; Lee，P.8; Rau，C.7; Robinsona，I. K.2,9; Yusufa，M.10

2020

10.1107/S1600577519015583

JOURNAL OF SYNCHROTRON RADIATION   影响因子和分区

0909-0495

1600-5775

Advanced imaging is useful for understanding the three-dimensional (3D) growth of cells. X-ray tomography serves as a powerful noninvasive, nondestructive technique that can fulfill these purposes by providing information about cell growth within 3D platforms. There are a limited number of studies taking advantage of synchrotron X-rays, which provides a large field of view and suitable resolution to image cells within specific biomaterials. In this study, X-ray synchrotron radiation microtomography at Diamond Light Source and advanced image processing were used to investigate cellular infiltration of HeLa cells within poly l-lactide (PLLA) scaffolds. This study demonstrates that synchrotron X-rays using phase contrast is a useful method to understand the 3D growth of cells in PLLA electrospun scaffolds. Two different fiber diameter (2 and 4μm) scaffolds with different pore sizes, grown over 2, 5 and 8 days in vitro, were examined for infiltration and cell connectivity. After performing visualization by segmentation of the cells from the fibers, the results clearly show deeper cell growth and higher cellular interconnectivity in the 4μm fiber diameter scaffold. This indicates the potential for using such 3D technology to study cell-scaffold interactions for future medical use.

3D culture electrospun polymer scaffold image analysis synchrotron X-ray CT

SCI ; EI

英语

其他

National Natural Science Foundation of China[11775105]

Instruments & Instrumentation ; Optics ; Physics

Instruments & Instrumentation ; Optics ; Physics, Applied

WOS:000505777800020

International Union of Crystallography, 5 Abbey Square, Chester, CH1 2HU, United Kingdom

20200107971907

Cell growth ; Cell proliferation ; Computerized tomography ; Cytology ; Growth kinetics ; Image analysis ; Lanthanum compounds ; Light sources ; Pore size ; Synchrotron radiation ; Synchrotrons ; X rays

Biomedical Engineering:461.1 ; Biology:461.9 ; Computer Applications:723.5 ; High Energy Physics:932.1 ; Particle Accelerators:932.1.1 ; Materials Science:951

PHYSICS

2-s2.0-85077170778

Scopus

1.London Centre for Nanotechnology,University College London,London,WC1H 0AH,United Kingdom
2.Research Complex at Harwell Rutherford Appleton Laboratory,Didcot,OX11 0FA,United Kingdom
3.3DMagination Ltd,Atlas Building,Fermi Avenue,Harwell,Didcot OX11 0QX,United Kingdom
4.School of Mechanical,Aerospace and Civil Engineering,University of Manchester,Manchester,United Kingdom
5.Department of Applied Computing,University of Buckingham,United Kingdom
6.Department of Electrical and Electronic Engineering,Southern University of Science and Technology,Shenzhen,518055,China
7.Diamond Light Source,Oxfordshire OX11 0DE,United Kingdom
8.Mechanical Engineering,University College London,London,WC1E 7JE,United Kingdom
9.Condensed Matter Physics and Materials Department,Brookhaven National Laboratory,Upton,11973,United States
10.Centre for Regenerative Medicine and Stem Cell Research,Aga Khan University,Karachi,Pakistan

Bhartiya，A.,Madi，K.,Disney，C. M.,et al. Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays Bhartiya A.[J]. JOURNAL OF SYNCHROTRON RADIATION,2020,27:158-163.

Bhartiya，A..,Madi，K..,Disney，C. M..,Courtois，L..,Jupe，A..,...&Yusufa，M..(2020).Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays Bhartiya A..JOURNAL OF SYNCHROTRON RADIATION,27,158-163.

Bhartiya，A.,et al."Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays Bhartiya A.".JOURNAL OF SYNCHROTRON RADIATION 27(2020):158-163.