Transcriptome dynamics of the BHK21 cell line in response to human coronavirus OC43 infection

Li，Mianhuan1,2; Yang，Yang2; Wang，Pusen2; Que，Weitao2; Zhong，Lin2; Cai，Zhao3; Liu，Yang4; Yang，Liang1,2; Liu，Yingxia2

2024-08-01

10.1016/j.micres.2024.127750

Microbiological Research   影响因子和分区

0944-5013

The progress of viral infection involves numerous transcriptional regulatory events. The identification of the newly synthesized transcripts helps us to understand the replication mechanisms and pathogenesis of the virus. Here, we utilized a time-resolved technique called metabolic RNA labeling approach called thiol(SH)-linked alkylation for the metabolic sequencing of RNA (SLAM-seq) to differentially elucidate the levels of steady-state and newly synthesized RNAs of BHK21 cell line in response to human coronavirus OC43 (HCoV-OC43) infection. Our results showed that the Wnt/β-catenin signaling pathway was significantly enriched with the newly synthesized transcripts of BHK21 cell line in response to HCoV-OC43 infection. Moreover, inhibition of the Wnt pathway promoted viral replication in the early stage of infection, but inhibited it in the later stage of infection. Furthermore, remdesivir inhibits the upregulation of the Wnt/β-catenin signaling pathway induced by early infection with HCoV-OC43. Collectively, our study showed the diverse roles of Wnt/β-catenin pathway at different stages of HCoV-OC43 infection, suggesting a potential target for the antiviral treatment. In addition, although infection with HCoV-OC43 induces cytopathic effects in BHK21 cells, inhibiting apoptosis does not affect the intracellular replication of the virus. Monitoring newly synthesized RNA based on such time-resolved approach is a highly promising method for studying the mechanism of viral infections.

HCoV-OC43 Introduction Remdesivir SLAM-seq Viral infection Wnt pathway

EI

英语

第一 ; 通讯

20242116116232

Cell culture ; Cell death ; Cell signaling ; Metabolism ; RNA

Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biology:461.9

MICROBIOLOGY

2-s2.0-85193433409

Scopus

1.School of Medicine,Southern University of Science and Technology,Shenzhen,518055,China
2.Shenzhen Third People's Hospital,National Clinical Research Centre for Infectious Disease,The Second Affiliated Hospital of Southern University of Science and Technology,Shenzhen,518112,China
3.Shenzhen Mindray Bio-Medical Electronics Co.,Ltd,Shenzhen,518057,China
4.Southern University of Science and Technology Hospital,Shenzhen,518055,China

Li，Mianhuan,Yang，Yang,Wang，Pusen,et al. Transcriptome dynamics of the BHK21 cell line in response to human coronavirus OC43 infection[J]. Microbiological Research,2024,285.

Li，Mianhuan.,Yang，Yang.,Wang，Pusen.,Que，Weitao.,Zhong，Lin.,...&Liu，Yingxia.(2024).Transcriptome dynamics of the BHK21 cell line in response to human coronavirus OC43 infection.Microbiological Research,285.

Li，Mianhuan,et al."Transcriptome dynamics of the BHK21 cell line in response to human coronavirus OC43 infection".Microbiological Research 285(2024).