IKVAV functionalized oriented PCL/Fe3O4 scaffolds for magnetically modulating DRG growth behavior

Liu，Yaqiong1,2; Gao，Hongxia1; Shang，Yuqing1; Sun，Shaolan1; Guan，Wenchao1; Zheng，Tiantian1; Wu，Linliang1,3; Cong，Meng1; Zhang，Luzhong1; Li，Guicai1,2

2024-07-01

10.1016/j.colsurfb.2024.113967

Colloids and Surfaces B: Biointerfaces   影响因子和分区

0927-7765

1873-4367

The re-bridging of the deficient nerve is the main problem to be solved after the functional impairment of the peripheral nerve. In this study, a directionally aligned polycaprolactone/triiron tetraoxide (PCL/FeO) fiber scaffolds were firstly prepared by electrospinning technique, and further then grafted with IKVAV peptide for regulating DRG growth and axon extension in peripheral nerve regeneration. The results showed that oriented aligned magnetic PCL/FeO composite scaffolds were successfully prepared by electrospinning technique and possessed good mechanical properties and magnetic responsiveness. The PCL/FeO scaffolds containing different FeO concentrations were free of cytotoxicity, indicating the good biocompatibility and low cytotoxicity of the scaffolds. The IKVAV-functionalized PCL/FeO scaffolds were able to guide and promote the directional extension of axons, the application of external magnetic field and the grafting of IKVAV peptides significantly further promoted the growth of DRGs and axons. The ELISA test results showed that the AP-10 F group scaffolds promoted the secretion of nerve growth factor (NGF) from DRG under a static magnetic field (SMF), thus promoting the growth and extension of axons. Importantly, the IKVAV-functionalized PCL/FeO scaffolds could significantly up-regulate the expression of Cntn2, PCNA, Sox10 and Isca1 genes related to adhesion, proliferation and magnetic receptor function under the stimulation of SMF. Therefore, IKVAV-functionalized PCL/FeO composite oriented scaffolds have potential applications in neural tissue engineering.

Axon extension Electrospinning Magnetic nanoparticles Oriented scaffolds Polycaprolactone

SCI ; EI

英语

通讯

20242116109626

Biocompatibility ; Biomechanics ; Magnetic fields ; Magnetite ; Nanomagnetics ; Peptides ; Scaffolds ; Scaffolds (biology) ; Tissue

Construction Equipment:405.1 ; Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Biomechanics, Bionics and Biomimetics:461.3 ; Biology:461.9 ; Immunology:461.9.1 ; Magnetism: Basic Concepts and Phenomena:701.2 ; Fiber Chemistry and Processing:819.3

BIOLOGY & BIOCHEMISTRY

2-s2.0-85193263768

Scopus

1.Key Laboratory of Neuroregeneration,Co-Innovation Center of Neuroregeneration,Nantong University,Nantong,226001,China
2.Guangdong Provincial Key Laboratory of Advanced Biomaterials,Southern University of Science and Technology,Shenzhen,518055,China
3.The People's Hospital of Rugao,Affiliated Hospital of Nantong University,Nantong,226599,China

Liu，Yaqiong,Gao，Hongxia,Shang，Yuqing,et al. IKVAV functionalized oriented PCL/Fe3O4 scaffolds for magnetically modulating DRG growth behavior[J]. Colloids and Surfaces B: Biointerfaces,2024,239.

Liu，Yaqiong.,Gao，Hongxia.,Shang，Yuqing.,Sun，Shaolan.,Guan，Wenchao.,...&Li，Guicai.(2024).IKVAV functionalized oriented PCL/Fe3O4 scaffolds for magnetically modulating DRG growth behavior.Colloids and Surfaces B: Biointerfaces,239.

Liu，Yaqiong,et al."IKVAV functionalized oriented PCL/Fe3O4 scaffolds for magnetically modulating DRG growth behavior".Colloids and Surfaces B: Biointerfaces 239(2024).