Cryomicroneedle delivery of nanogold-engineered Rhodospirillum rubrum for photochemical transformation and tumor optical biotherapy

Shi，Qingxia1,2; Yin，Ting2; Zeng，Cuiping3; Pan，Hong1; Chen，Ze1; Wang，Lin3; Wang，Bo3; Zheng，Mingbin1,2,4; Cai，Lintao1,5

2024-07-01

10.1016/j.bioactmat.2024.03.032

Bioactive Materials   影响因子和分区

2452-199X

Tumor metabolite regulation is intricately linked to cancer progression. Because lactate is a characteristic metabolite of the tumor microenvironment (TME), it supports tumor progression and drives immunosuppression. In this study, we presented a strategy for antitumor therapy by developing a nanogold-engineered Rhodospirillum rubrum (R.r-Au) that consumed lactate and produced hydrogen for optical biotherapy. We leveraged a cryogenic micromolding approach to construct a transdermal therapeutic cryomicroneedles (CryoMNs) patch integrated with R.r-Au to efficiently deliver living bacterial drugs. Our long-term storage studies revealed that the viability of R.r-Au in CryoMNs remained above 90%. We found that the CryoMNs patch was mechanically strong and could be inserted into mouse skin. In addition, it rapidly dissolved after administering bacterial drugs and did not produce by-products. Under laser irradiation, R.r-Au effectively enhanced electron transfer through Au NPs actuation into the photosynthetic system of R. rubrum and enlarged lactate consumption and hydrogen production, thus leading to an improved tumor immune activation. Our study demonstrated the potential of CryoMNs-R.r-Au patch as a minimally invasive in situ delivery approach for living bacterial drugs. This research opens up new avenues for nanoengineering bacteria to transform tumor metabolites into effective substances for tumor optical biotherapy.

Cryomicroneedles Hydrogen generation Lactate depletion Optical biotherapy Photosynthetic bacteria

SCI

英语

其他

2-s2.0-85190953049

Scopus

1.Guangdong Key Laboratory of Nanomedicine,CAS-HK Joint Lab of Biomaterials,Shenzhen Engineering Laboratory of Nanomedicine and Nanoformulations,Institute of Biomedicine and Biotechnology,Shenzhen Institute of Advanced Technology (SIAT),Chinese Academy of Sciences,Shenzhen,518055,China
2.Guangdong Key Laboratory for Research and Development of Natural Drugs,Key Laboratory for Nanomedicine,Guangdong Medical University,Dongguan,523808,China
3.CAS Key Laboratory of Quantitative Engineering Biology Shenzhen Institute of Synthetic Biology Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences,Shenzhen,518055,China
4.National Clinical Research Center for Infectious Disease,Shenzhen Third People's Hospital,The Second Affiliated Hospital,Southern University of Science and Technology,Shenzhen,518112,China
5.Sino-Euro Center of Biomedicine and Health,Shenzhen,518024,China

Shi，Qingxia,Yin，Ting,Zeng，Cuiping,et al. Cryomicroneedle delivery of nanogold-engineered Rhodospirillum rubrum for photochemical transformation and tumor optical biotherapy[J]. Bioactive Materials,2024,37:505-516.

Shi，Qingxia.,Yin，Ting.,Zeng，Cuiping.,Pan，Hong.,Chen，Ze.,...&Cai，Lintao.(2024).Cryomicroneedle delivery of nanogold-engineered Rhodospirillum rubrum for photochemical transformation and tumor optical biotherapy.Bioactive Materials,37,505-516.

Shi，Qingxia,et al."Cryomicroneedle delivery of nanogold-engineered Rhodospirillum rubrum for photochemical transformation and tumor optical biotherapy".Bioactive Materials 37(2024):505-516.