Perfluoroalkyl sulfonate induces cardiomyocyte apoptosis via endoplasmic reticulum stress activation and autophagy flux inhibition

Wang，Yuanhao1; Yin，Da2; Sun，Xin2; Zhang，Wei1; Ma，Huan3; Huang，Jingnan1; Yang，Chuanbin1; Wang，Jigang1,4; Geng，Qingshan1

2024-06-20

10.1016/j.scitotenv.2024.172582

Science of the Total Environment   影响因子和分区

0048-9697

1879-1026

Perfluoroalkyl sulfonate (PFOS) is a commonly used chemical compound that often found in materials such as waterproofing agents, food packaging, and fire retardants. Known for its stability and persistence in the environment, PFOS can enter the human body through various pathways, including water and the food chain, raising concerns about its potential harm to human health. Previous studies have suggested a cardiac toxicity of PFOS, but the specific cellular mechanisms remained unclear. Here, by using AC16 cardiomyocyte as a model to investigate the molecular mechanisms potential the cardiac toxicity of PFOS. Our findings revealed that PFOS exposure reduced cell viability and induces apoptosis in human cardiomyocyte. Proteomic analysis and molecular biological techniques showed that the Endoplasmic Reticulum (ER) stress-related pathways were activated, while the cellular autophagy flux was inhibited in PFOS-exposed cells. Subsequently, we employed strategies such as autophagy activation and ER stress inhibition to alleviate the PFOS-induced apoptosis in AC16 cells. These results collectively suggest that PFOS-induced ER stress activation and autophagy flux inhibition contribute to cardiomyocyte apoptosis, providing new insights into the mechanisms of PFOS-induced cardiomyocyte toxicity.

Autophagy Cardiomyocyte Cardiotoxicity ER stress Perfluoroalkyl sulfonate

SCI ; EI

英语

第一 ; 通讯

ENVIRONMENT/ECOLOGY

2-s2.0-85191521785

Scopus

1.Department of Geriatrics,Shenzhen Clinical Research Centre for Geriatrics,Shenzhen People's Hospital,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,Guangdong,518020,China
2.Department of Cardiology,Shenzhen Cardiovascular Minimally Invasive Medical Engineering Technology Research and Development Center,Shenzhen People's Hospital,The First Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,518020,China
3.Guangdong Cardiovascular Institute,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou,No.106 Zhongshan Er Road, Guangdong,China
4.State Key Laboratory for Quality Esurance and Sustainable Use of Dao-di Herbs,Artemisinin Research Center,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,100700,China

Wang，Yuanhao,Yin，Da,Sun，Xin,et al. Perfluoroalkyl sulfonate induces cardiomyocyte apoptosis via endoplasmic reticulum stress activation and autophagy flux inhibition[J]. Science of the Total Environment,2024,930.

Wang，Yuanhao.,Yin，Da.,Sun，Xin.,Zhang，Wei.,Ma，Huan.,...&Geng，Qingshan.(2024).Perfluoroalkyl sulfonate induces cardiomyocyte apoptosis via endoplasmic reticulum stress activation and autophagy flux inhibition.Science of the Total Environment,930.

Wang，Yuanhao,et al."Perfluoroalkyl sulfonate induces cardiomyocyte apoptosis via endoplasmic reticulum stress activation and autophagy flux inhibition".Science of the Total Environment 930(2024).