3D drift correction for super-resolution imaging with a single laser light

Li，Yunze; He，Yingchuan; Fang，Ke; Zhou，Lulu; Wang，Zhen; Shi，Wei; Li，Yiming

2024-05-15

10.1364/OL.519290

Optics Letters   影响因子和分区

0146-9592

1539-4794

Single-molecule localization microscopy (SMLM) enables three-dimensional (3D) super-resolution imaging of nanoscale structures within biological samples. However, prolonged acquisition introduces a drift between the sample and the imaging system, resulting in artifacts in the reconstructed super-resolution image. Here, we present a novel, to our knowledge, 3D drift correction method that utilizes both the reflected and scattered light from the sample. Our method employs the reflected light of a near-infrared (NIR) laser for focus stabilization while synchronously capturing speckle images to estimate the lateral drift. This approach combines high-precision active compensation in the axial direction with lateral post-processing compensation, achieving the abilities of 3D drift correction with a single laser light. Compared to the popular localization events-based cross correlation method, our approach is much more robust, especially for datasets with sparse localization points.

SCI ; EI

英语

第一

PHYSICS

2-s2.0-85193205740

Scopus

Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China

Li，Yunze,He，Yingchuan,Fang，Ke,et al. 3D drift correction for super-resolution imaging with a single laser light[J]. Optics Letters,2024,49(10):2785-2788.

Li，Yunze.,He，Yingchuan.,Fang，Ke.,Zhou，Lulu.,Wang，Zhen.,...&Li，Yiming.(2024).3D drift correction for super-resolution imaging with a single laser light.Optics Letters,49(10),2785-2788.

Li，Yunze,et al."3D drift correction for super-resolution imaging with a single laser light".Optics Letters 49.10(2024):2785-2788.