基于OCT检测的光生物调节对脑血液微循环的作用研究

全球老龄化是一个不可避免的现实，并带来了一系列健康问题，其中脑血管疾病尤为显著。鉴于大脑是高度血管化的器官，随着年龄增长及其他风险因素的积累，血管状态会受到影响进而导致血液微循环障碍，加速 脑血管疾病的进程。因此，对于脑血管疾病的预防和治疗显得非常迫切。目前，光生物调节作为一种新型非侵入式治疗技术，因其低成本和有效改善脑血液循环的潜力，在脑血管疾病的预防和治疗中展现出广阔的应用前景。然而，受限于分辨率和光生物调节参数的不确定性，目前尚无法精确观测到特定参数对脑动静脉结构及血流变化的独立影响。为了从微观 层面深入探究光生物调节对脑血管网络的作用机制，本研究提出了利用具 有微米级别分辨率的光学相干层析成像技术，监测不同参数下短期光生物调节对小鼠大脑皮层动静脉血流动态变化的影响。本课题比较了不同波长激光、连续照射时长及照射方式在调节脑血液 循环作用方面的差异。具体的，研究了中心波长为 650 nm，780 nm，808 nm 和 980 nm 照射30 分钟的调节作用。此外，以 808 nm为光源，对比了 30 分钟和1小时连续照射调节的差异，并分别评估了30分钟连续照射和脉 冲照射的效果。实验结果表明，使用波长808 nm，照射时间为30分钟的连续光生物调 节会对小鼠大脑动脉平均血流量产生最大值为 51%的促进作用，照射时间 的延长和40 Hz的脉冲式照射方式都没有达到相同的响应强度。650 nm 和 780 nm实验促进静脉血流作用显著，650 nm静脉平均血流量增加34%，780 nm 静脉平均血流量增加18%。这些都说明650 nm、780 nm和808 nm的30 分钟光生物调节有助于改善局部脑血液循环。本课题探究了短期光生物调节结合光学相干断层扫描技术在小鼠清醒 状态下的脑血液循环变化，表明了光生物调节在小鼠脑血管及脑血液循环 的影响中具有显著效果和安全性。

Global aging is an inevitable reality that has brought a series of health issues, among which cerebrovascular diseases are particularly severe. The brain is a highly vascularized organ, and with age and the accumulation of other risk factors, vascular health is affected and lead to microcirculation disorders, accelerating the progression of cerebrovascular diseases. Therefore, the prevention and treatment of cerebrovascular diseases are particularly urgent.Photobiomodulation (PBM) is a new non-invasive treatment technique, it is considered as a potential approach for the prevention and treatment of cerebrovascular diseases technology. Due to its low cost and effective improvement in cerebral blood circulation, this technology is highly anticipated. However, due to limitations in resolution and uncertainty in PBM parameters, it is currently impossible to accurately observe the effects of specific parameters on the structure and blood flow changes of cerebral arteries and veins. To understand the effect of PBM on the cerebral vascular network at the micro level, this study proposes the use of optical coherence tomography (OCT) with micrometer-level resolution to monitor the dynamic changes in cerebral cortex arteriovenous blood flow in mice under different parameters of short-term PBM.This study has compared the differences in the regulation of cerebral microcirculation by different laser wavelengths, continuous irradiation durations, and irradiation modes. Specifically, the study has examined the regulatory effects of different wavelengths (650 nm, 780 nm, 808 nm, and 980 nm) from the laser source, also has compared the differences between 30 minutes and one-hour continuous irradiation modulation, and has contrasted the effects of continuous irradiation versus pulsed irradiation.The experimental results show that continuous PBM with a wavelength of 808 nm and an irradiation time of 30 minutes have the greatest promotional effect on the average blood flow in the mouse brain arteries, with a maximum value of 51%. The extension of irradiation time and 40 Hz pulsed regulation do not achieve the same response intensity. Experiments with 650 nm and 780 nm significantly promoted venous blood flow, with an average increase in blood flow of 34% for 650 nm and 18% for 780 nm. This indicates that 30 minutes of PBM at 650 nm, 780 nm, and 808 nm helps improve local cerebral blood circulation.This study has investigated the effects of short-term PBM combined with OCT on cerebral blood circulation in awake mice. The results demonstrate that PBM technology has significant effects and safety in the impact on mouse cerebral vessels and cerebral blood circulation.

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