A method of genetic transformation and gene editing of succulents without tissue culture

Lu，Jinghua1,2,3; Li，Shanshan4; Deng，Shuai5; Wang，Mugui4,6; Wu，Yinghuang4; Li，Ming4,6; Dong，Jinsong4,6; Lu，Suhui5; Su，Chunli5; Li，Guofu5; Lang，Zhaobo3; Zhu，Jian Kang3,7

2024-07-01

10.1111/pbi.14318

Plant Biotechnology Journal   影响因子和分区

1467-7644

1467-7652

Succulents, valued for their drought tolerance and ornamental appeal, are important in the floriculture market. However, only a handful of succulent species can be genetically transformed, making it difficult to improve these plants through genetic engineering. In this study, we adapted the recently developed cut-dip-budding (CDB) gene delivery system to transform three previously recalcitrant succulent varieties – the dicotyledonous Kalanchoe blossfeldiana and Crassula arborescens and the monocotyledonous Sansevieria trifasciata. Capitalizing on the robust ability of cut leaves to regenerate shoots, these plants were successfully transformed by directly infecting cut leaf segments with the Agrobacterium rhizogenes strain K599. The transformation efficiencies were approximately 74%, 5% and 3.9%–7.8%, respectively, for K. blossfeldiana and C. arborescens and S. trifasciata. Using this modified CDB method to deliver the CRISPR/Cas9 construct, gene editing efficiency in K. blossfeldiana at the PDS locus was approximately 70%. Our findings suggest that succulents with shoot regeneration ability from cut leaves can be genetically transformed using the CDB method, thus opening up an avenue for genetic engineering of these plants.

cut–dip–budding (CDB) delivery system gene editing genetic transformation succulents

SCI

英语

其他

PLANT & ANIMAL SCIENCE

2-s2.0-85186943615

Scopus

1.Sanya Research Institute,Chinese Academy of Tropical Agricultural Sciences,Sanya,China
2.Hainan Yazhou Bay Seed Laboratory,Sanya,China
3.Institute of Advanced Biotechnology and School of Medicine,Southern University of Science and Technology,Shenzhen,China
4.Institute of Crop Sciences/National Nanfan Research Institute,Chinese Academy of Agricultural Sciences (CAAS),Sanya,China
5.Shandong Shunfeng Biotechnology Co. Ltd.,Jinan,China
6.Key Laboratory of Gene Editing Technologies (Hainan),Ministry of Agriculture and Rural Affairs,Sanya,China
7.Center for Advanced Bioindustry Technologies,Chinese Academy of Agricultural Sciences (CAAS),Beijing,China

Lu，Jinghua,Li，Shanshan,Deng，Shuai,et al. A method of genetic transformation and gene editing of succulents without tissue culture[J]. Plant Biotechnology Journal,2024,22(7):1981-1988.

Lu，Jinghua.,Li，Shanshan.,Deng，Shuai.,Wang，Mugui.,Wu，Yinghuang.,...&Zhu，Jian Kang.(2024).A method of genetic transformation and gene editing of succulents without tissue culture.Plant Biotechnology Journal,22(7),1981-1988.

Lu，Jinghua,et al."A method of genetic transformation and gene editing of succulents without tissue culture".Plant Biotechnology Journal 22.7(2024):1981-1988.