南方科技大学知识苑(SUSTech KC): An Artificial Enzyme for Asymmetric Nitrocyclopropanation of α,β-Unsaturated Aldehydes

题名	An Artificial Enzyme for Asymmetric Nitrocyclopropanation of α,β-Unsaturated Aldehydes—Design and Evolution
作者	Yu，Ming Zhu 1; Yuan，Ye 1; Li，Zhen Jie2 ; Kunthic，Thittaya 1; Wang，He Xiang 1; Xu，Chen2 ; Xiang，Zheng 1,3
通讯作者	Xiang，Zheng
发表日期	2024-06-17
DOI	10.1002/anie.202401635
发表期刊	Angewandte Chemie - International Edition 影响因子和分区
ISSN	1433-7851
EISSN	1521-3773
卷号	63 期号:25
摘要	The introduction of an abiological catalytic group into the binding pocket of a protein host allows for the expansion of enzyme chemistries. Here, we report the generation of an artificial enzyme by genetic encoding of a non-canonical amino acid that contains a secondary amine side chain. The non-canonical amino acid and the binding pocket function synergistically to catalyze the asymmetric nitrocyclopropanation of α,β-unsaturated aldehydes by the iminium activation mechanism. The designer enzyme was evolved to an optimal variant that catalyzes the reaction at high conversions with high diastereo- and enantioselectivity. This work demonstrates the application of genetic code expansion in enzyme design and expands the scope of enzyme-catalyzed abiological reactions.
关键词	artificial enzyme asymmetric cyclopropanation biocatalysis directed evolution non-canonical amino acid
相关链接	[Scopus记录]
收录类别	SCI ; EI
语种	英语
学校署名	其他
ESI学科分类	CHEMISTRY
Scopus记录号	2-s2.0-85193026122
来源库	Scopus
引用统计	被引频次[WOS]：6
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/778709
专题	理学院_化学系深圳格拉布斯研究院
作者单位	1.State Key Laboratory of Chemical Oncogenomics,Guangdong Provincial Key Laboratory of Chemical Genomics,AI for Science (AI4S) Preferred Program,School of Chemical Biology and Biotechnology Peking University Shenzhen Graduate School,University Town of Shenzhen,Nanshan District,Shenzhen,518055,China 2.Shenzhen Grubbs Institute and Department of Chemistry,Guangdong Provincial Key Laboratory of Catalysis,Southern University of Science and Technology Nanshan District,Shenzhen,518055,China 3.Institute of Chemical Biology,Shenzhen Bay Laboratory Gaoke Innovation Center,Shenzhen,Guangqiao Road, Guangming District,518132,China
推荐引用方式 GB/T 7714	Yu，Ming Zhu,Yuan，Ye,Li，Zhen Jie,等. An Artificial Enzyme for Asymmetric Nitrocyclopropanation of α,β-Unsaturated Aldehydes—Design and Evolution[J]. Angewandte Chemie - International Edition,2024,63(25).
APA	Yu，Ming Zhu.,Yuan，Ye.,Li，Zhen Jie.,Kunthic，Thittaya.,Wang，He Xiang.,...&Xiang，Zheng.(2024).An Artificial Enzyme for Asymmetric Nitrocyclopropanation of α,β-Unsaturated Aldehydes—Design and Evolution.Angewandte Chemie - International Edition,63(25).
MLA	Yu，Ming Zhu,et al."An Artificial Enzyme for Asymmetric Nitrocyclopropanation of α,β-Unsaturated Aldehydes—Design and Evolution".Angewandte Chemie - International Edition 63.25(2024).