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题名

HNRNP H1 / H2 / F 在 B 细胞早期发育中 的作用及机制研究

其他题名
RESEARCH ON CORPORATE ESG RATING INCENTIVE MECHANISM FROM THE PERSPECTIVE OF SOCIAL WELFARE
姓名
姓名拼音
MENG Limin
学号
12133058
学位类型
硕士
学位专业
0710 生物学
学科门类/专业学位类别
07 理学
导师
欧西军
导师单位
基础免疫与微生物学系
论文答辩日期
2024-05
论文提交日期
2024-07-02
学位授予单位
南方科技大学
学位授予地点
深圳
摘要
成人 B 细胞始于造血干细胞,在骨髓中发育并迁移到外周淋巴器官分
化 成 熟 发 挥 免 疫 功 能 。 异 质 核 糖 核 蛋 白 ( heterogeneous nuclear ribonucleoproteinshnRNPH F 是一类介导转录后调控的重要 RNA 结合蛋白,主要参与核酸的代谢调节,且 hnRNP H hnRNP F 由于其结构和
功能高度相似通常共同发挥调控作用。目前对 hnRNP H/F 的研究多见于其
与肿瘤、神经元相关疾病的联系,然而,hnRNP H/F 在早期 B 淋巴细胞发
育中的生理功能尚不清楚。本研究中,利用 Cre-LoxP 系统构建了在 B 细胞
特异性敲除 hnRNP H1H2 F 的小鼠,我们发现 hnRNP H1/H2/F B
胞的早期发育至关重要。hnRNP H1H2 F 的缺失导致小鼠 B 细胞发育
被阻滞在 pre-pro-B 阶段,骨髓和脾脏中 B 细胞的数量和比例急剧减少,进
一步分析发现 B 细胞的减少与细胞凋亡增加有关。机制上,我们发现缺失
hnRNP H1/H2/F 使得小鼠 Ig 重链的 VDJ 重排受损,细胞内 Ig 重链蛋白(μ
chain)表达降低。通过 RNA-seq RT-qPCR,我们发现在 hnRNP H1/H2/F
缺失的 B 细胞中,包括 E2aEbf1 Pax5 在内的 B 细胞谱系决定基因的表
达显著下调。综上,hnRNP H1/H2/F 可能通过调控 B 细胞谱系决定基因的
表达来促进 B 细胞的早期发育。
关键词
语种
中文
培养类别
独立培养
入学年份
2021
学位授予年份
2024-07
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所在学位评定分委会
生物学
国内图书分类号
Q939.91
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条目标识符http://sustech.caswiz.com/handle/2SGJ60CL/778871
专题生命科学学院_生物系
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孟丽敏. HNRNP H1 / H2 / F 在 B 细胞早期发育中 的作用及机制研究[D]. 深圳. 南方科技大学,2024.
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