南方科技大学知识苑(SUSTech KC): Inhibition of CSPG-PTPÏ Activates Autophagy Flux and Lysosome Fusion, Aids Axon and Synaptic Reorganization in Spinal Cord Injury

题名	Inhibition of CSPG-PTPÏ Activates Autophagy Flux and Lysosome Fusion, Aids Axon and Synaptic Reorganization in Spinal Cord Injury
作者	Wang, Hongyu 1,2,3,8 ; Feng, Naibo 1,2; Liu, Chungeng 1,2; Xie, Yongheng 1,2; Zhou, Zipeng 4; Zhao, Haosen 5; Xiao, Guozhi6,7 ; Yang, Dazhi 1,2,3,8
通讯作者	Wang, Hongyu; Yang, Dazhi
发表日期	2024-06-01
DOI	10.1007/s12035-024-04304-3
发表期刊	MOLECULAR NEUROBIOLOGY 影响因子和分区
ISSN	0893-7648
EISSN	1559-1182
摘要	Chondroitin sulfate proteoglycans (CSPGs) and proteoglycan receptor protein tyrosine phosphatase sigma (PTP sigma) play a critical role in the pathology of spinal cord injury (SCI). CSPGs can be induced by autophagy inhibition in astrocyte. However, CSPG's impact on autophagy and its role in SCI is still unknown. We investigate intracellular sigma peptide (ISP) targeting PTP sigma, its effects on autophagy, and synaptic reorganization in SCI. We found that ISP increased the level of autophagosome marker LC3B-II/I and decreased autophagosome degradation marker p62 in SCI, suggesting activated autophagy flux. ISP restored autophagosome-lysosome fusion-related protein syntaxin 17 (STX17) and lysosome-associated membrane protein 2 (LAMP2), indicating activated autophagosome-lysosome fusion. ISP increased pre-synaptic marker synaptophysin (SYN) and postsynaptic density protein-95 (PSD-95) expression and improved excitatory synapse marker vesicular glutamate transporter 1 (VGLUT1) and SYN in SCI, suggesting improved synaptic reorganization. ISP promoted axon marker neurofilament and growth-related GAP-43 expression in SCI. ISP rescued a preserved number of motor neurons and improved neurobehavioral recovery after SCI. Our study extended the CSPG-PTP sigma inhibition role in activating autophagy flux, axon and synaptic reorganization, and functional recovery in SCI.
关键词	Spinal cord injury PTP sigma CSPG Autophagy flux Lysosome fusion
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
WOS记录号	WOS:001251244100002
出版者	SPRINGER
ESI学科分类	NEUROSCIENCE & BEHAVIOR
来源库	Web of Science
引用统计
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/778963
专题	南方科技大学第一附属医院南方科技大学医学院南方科技大学医学院_生物化学系
作者单位	1.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Shenzhen 518055, Peoples R China 2.Shenzhen Key Lab Reconstruct Struct & Funct Sports, Shenzhen 518000, Guangdong, Peoples R China 3.Jinan Univ, Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1,Clin Med Coll 2,Dept Geriat, Shenzhen 518109, Guangdong, Peoples R China 4.Jinzhou Med Univ, Dept Orthoped Surg, Affiliated Hosp 1, Jinzhou 121000, Liaoning, Peoples R China 5.Jinzhou Med Univ, Dept Rehabil, Affiliated Hosp 3, Jinzhou 121000, Liaoning, Peoples R China 6.Southern Univ Sci & Technol, Sch Med, Dept Biochem, Shenzhen Key Lab Cell Microenvironm, Shenzhen, Peoples R China 7.Southern Univ Sci & Technol, Sch Med, Guangdong Prov Key Lab Cell Microenvironm & Dis Re, Shenzhen, Peoples R China 8.Shenzhen Peoples Hosp, Dept Orthoped Surg, Shenzhen 518000, Guangdong, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Wang, Hongyu,Feng, Naibo,Liu, Chungeng,et al. Inhibition of CSPG-PTPÏ Activates Autophagy Flux and Lysosome Fusion, Aids Axon and Synaptic Reorganization in Spinal Cord Injury[J]. MOLECULAR NEUROBIOLOGY,2024.
APA	Wang, Hongyu.,Feng, Naibo.,Liu, Chungeng.,Xie, Yongheng.,Zhou, Zipeng.,...&Yang, Dazhi.(2024).Inhibition of CSPG-PTPÏ Activates Autophagy Flux and Lysosome Fusion, Aids Axon and Synaptic Reorganization in Spinal Cord Injury.MOLECULAR NEUROBIOLOGY.
MLA	Wang, Hongyu,et al."Inhibition of CSPG-PTPÏ Activates Autophagy Flux and Lysosome Fusion, Aids Axon and Synaptic Reorganization in Spinal Cord Injury".MOLECULAR NEUROBIOLOGY (2024).