Deciphering the adaptation mechanism of anammox consortia under sulfamethoxazole stress: A model coupling resistance accumulation and interspecies-cooperation

Nie, Cailong1; Chen, Liming1; Zhao, Bixi1; Wu, Ziqi1; Zhang, Miao1; Yan, Yuxi1; Li, Bing4; Xia, Yu1,2,3

2024-09-05

10.1016/j.jhazmat.2024.135074

JOURNAL OF HAZARDOUS MATERIALS   影响因子和分区

0304-3894

1873-3336

strategy has not yet been fully addressed. Therefore, in this study, we initially ascertained anammox consortia's ability to co-metabolize SMX in batch tests. Then, a 200-day domestication process of anammox consortia under SMX stress was carried out with community variations and transcriptional activities monitored by metagenomic and metatranscriptomic sequencing techniques. Despite the initial drop to 41.88 %, the nitrogen removal efficiency of the anammox consortia rebounded to 84.64 % post-domestication under 5 mg/L SMX. Meanwhile, a 4.85-fold accumulation of antibiotic resistance genes (ARGs) under SMX stress was observed as compared to the control group. Interestingly, the anammox consortia may unlock the SMX-inhibited folate synthesis pathway through a novel interspecies cooperation triangle among Nitrospira (NAA), Desulfobacillus denitrificans (DSS1), and the core anammox population Candidatus Brocadia sinica (AMX1), in which the modified dihydropteroate synthase (encoded by sul1) of NAA reconnected the symbiotic cooperation between AMX1 and DSS1. Overall, this study provides a new model for the adaptation strategies of anammox consortia to SMX stress.

Anammox Sulfamethoxazole Metagenome Metatranscriptiome Interspecies cooperation Antibiotic resistance genes

SCI ; EI

英语

第一 ; 通讯

National Key R & D Program of China National[2022YFE0103200] ; Natural Science Foundation of China[42277103] ; Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control[2023B1212060002] ; Guangdong Provincial Key Laboratory of Environmental Health and Land Resource[2020B121201014] ; Shenzhen Science and Technology Innovation Committee[JCYJ20210324104412033]

Engineering ; Environmental Sciences & Ecology

Engineering, Environmental ; Environmental Sciences

WOS:001263512400001

ELSEVIER

20242716605360

Antibiotics ; Nitrogen removal ; Wastewater treatment

Industrial Wastes Treatment and Disposal:452.4 ; Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6

ENGINEERING

Web of Science

1.Southern Univ Sci & Technol, Coll Engn, Sch Environm Sci & Engn, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Sch Environm Sci & Engn, State Environm Protect Key Lab Integrated Surface, Sch Environm Sci & Engn, Shenzhen 518055, Peoples R China
3.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Guangdong Prov Key Lab Soil & Groundwater Pollut C, Shenzhen 518055, Peoples R China
4.Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch, State Environm Protect Key Lab Microorganism Appli, Beijing, Peoples R China

Nie, Cailong,Chen, Liming,Zhao, Bixi,et al. Deciphering the adaptation mechanism of anammox consortia under sulfamethoxazole stress: A model coupling resistance accumulation and interspecies-cooperation[J]. JOURNAL OF HAZARDOUS MATERIALS,2024,476.

Nie, Cailong.,Chen, Liming.,Zhao, Bixi.,Wu, Ziqi.,Zhang, Miao.,...&Xia, Yu.(2024).Deciphering the adaptation mechanism of anammox consortia under sulfamethoxazole stress: A model coupling resistance accumulation and interspecies-cooperation.JOURNAL OF HAZARDOUS MATERIALS,476.

Nie, Cailong,et al."Deciphering the adaptation mechanism of anammox consortia under sulfamethoxazole stress: A model coupling resistance accumulation and interspecies-cooperation".JOURNAL OF HAZARDOUS MATERIALS 476(2024).